LOCUS       sequence-197192-        8405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo GFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-GFP-2A-MCS
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..320
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     293..317
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             349..1044
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             1057..1113
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        1141..1651
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1570..1588
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1661..2257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1661..1680)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2157..2177
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2258..2311
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2312..2929,2930..2935,2936..2971,2972..2974,2975..3010,
                     3011..3013,3014..3049,3050..3058)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3098..3118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3516..3527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3732..3965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4037..4158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4074..4093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4128..4147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4198..4333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4333..4404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4405..4473,4474..5265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4623..4642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5436..6024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5925..5944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6178..6195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6312..6333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6348..6353,6354..6371,6372..6378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6386..6402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6391..6413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6410..6426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6410..6426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6445..6465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6447..6465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6493..6719
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6720..6900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6947..7072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7565..7798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7983..8027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8240..8357
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
       61 gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      181 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      241 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      301 tagtgaaccg tcagatcgcc tggagaattg gctagcgaat tcgccaccat ggagagcgac
      361 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
      421 gagttcgagc tggtgggcgg cggagagggc acccccgagc agggccgcat gaccaacaag
      481 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
      541 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
      601 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
      661 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtgatg
      721 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
      781 accgtggagc acctgcaccc catgggcgat aacgatctgg atggcagctt cacccgcacc
      841 ttcagcctgc gcgacggcgg ctactacagc tccgtggtgg acagccacat gcacttcaag
      901 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
      961 gaggaggatc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     1021 ccggatgcag atgccggtga agaaaccggt tccggagcca cgaacttctc tctgttaaag
     1081 caagcaggag acgtggaaga aaaccccggt cctaggctgc agacgcgttg tacaggatcc
     1141 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     1201 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     1261 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     1321 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     1381 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     1441 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     1501 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     1561 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     1621 cgttgaccga atcaccgacc tctctcccca gcaattcacc atgaccgagt acaagcccac
     1681 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     1741 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     1801 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     1861 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     1921 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     1981 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     2041 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     2101 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     2161 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     2221 accgcgcacc tggtgcatga cccgcaagcc cggtgccgaa ggtagaggtt ctctcctcac
     2281 ttgtggtgat gttgaagaaa accctggtcc aatgtctaga ctggacaaga gcaaagtcat
     2341 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     2401 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     2461 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     2521 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     2581 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     2641 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     2701 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     2761 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     2821 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     2881 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     2941 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     3001 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     3061 aagtaaggat cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg
     3121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3421 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3601 cgagtcggat ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga
     3661 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3721 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     3781 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3961 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4021 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4141 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     4201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4261 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4321 ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4381 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4441 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4501 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4561 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4621 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4681 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4741 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4801 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4861 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4921 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4981 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5041 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5101 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5161 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5221 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5281 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5341 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5401 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5461 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5521 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5581 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5641 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5701 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5761 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5821 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5881 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5941 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6001 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6061 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6121 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6181 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6241 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6301 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6361 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6421 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     6481 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     6541 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     6601 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     6661 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     6721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6901 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6961 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7021 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     7081 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     7141 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     7201 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     7261 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     7321 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7381 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     7441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     7681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     8041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     8101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     8161 cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa
     8221 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     8281 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     8341 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa
     8401 tcgat
//
