LOCUS       sequence-197286-        5069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ac transposase with SP6 promoter for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Chong-Morrison V, Mayes S, Simoes FC, Senanayake U, Carroll DS, 
            Riley PR, Wilson SW, Sauka-Spengler T
  TITLE     Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in 
            zebrafish.
  JOURNAL   Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. 
            Epub 2023 Jun 27.
  PUBMED    37367831
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2023-06-15"; volume: "12(6):bio059995. doi"; pages: "
            10.1242/bio.059995. Epub 2023 Jun 27"
COMMENT     Sequence Label: pAC-SP6
FEATURES             Location/Qualifiers
     source          1..5069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(9..597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(89..108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(768..1559,1560..1628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1391..1410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1629..1733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1801..1819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1857..1879)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1979..1998
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2212..2231
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2521..2539
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2521..2538
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           2551..2593
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      2601..2610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     3'UTR           4943..5068
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
       61 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1741 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1801 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     1861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     1981 gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg
     2041 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     2101 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     2161 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     2221 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     2281 ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg
     2341 cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt
     2401 ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta
     2461 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2521 atttaggtga cactatagaa tacaagcttg cttgttcttt ttgcagaagc tcagaataaa
     2581 cgctcaactt tggcagatcc gccaccatgg gtcctccaga gaagaagcgt aaggtagaaa
     2641 tggctattgt tcatgaacca caaccacaac cacaaccaca accagaacca caaccacagc
     2701 cacaacctga acccgaagaa gaagcaccac agaagagggc aaagaagtgc acatcggatg
     2761 tatggcagca tttcaccaag aaggaaattg aagtggaggt cgatggaaag aaatacgttc
     2821 aggtatgggg gcattgcaac tttcctaatt gcaaggctaa gtatagggct gagggtcatc
     2881 atggaacaag cggatttcga aatcacttga gaacatcaca tagtttagtt aaaggtcagt
     2941 tgtgtctaaa aagtgaaaag gatcatggca aagacataaa tctcattgag ccttataagt
     3001 acgatgaagt ggttagccta aagaagcttc atttggcaat aatcatgcat gaataccctt
     3061 tcaatattgt agaacatgag tactttgttg agtttgttaa gtctctgcgc cctcactttc
     3121 caataaagtc ccgtgtcact gctagaaaat atatcatgga tttgtatttg gaagaaaaag
     3181 aaaagttgta tggaaaacta aaagatgttc agtctcgctt cagtacaact atggatatgt
     3241 ggacatcttg tcaaaataag tcatacatgt gtgtcaccat ccattggatt gatgatgatt
     3301 ggtgtctcca aaaaagaatt gttggctttt ttcatgttga agggcgccac actggccaaa
     3361 ggttatcaca aaccttcact gcaatcatgg ttaagtggaa cattgagaaa aaattgtttg
     3421 ccttgtcttt ggataatgct agtgcaaatg aagtagctgt gcacgatata attgaggatt
     3481 tgcaggacac tgattcaaat ctagtttgtg atggtgcttt ctttcatgtg aggtgtgctt
     3541 gtcacatact gaacttggtt gcaaaggatg gcttggctgt aattgcagga acaattgaga
     3601 aaatcaaagc gattgttctt gctgtaaaat cttctccttt gcagtgggaa gaactaatga
     3661 agtgtgctag tgaatgtgac ttggataaat ctaaagggat ctcatatgat gtctcaacta
     3721 gatggaattc aacctatttg atgttgaggg atgccttata ttataagcct gcactaataa
     3781 ggcttaaaac aagtgatcct cgcaggtacg atgcaatttg tcctaaagcc gaggagtgga
     3841 agatggcatt aactcttttt aagtgtttga agaagttttt tgatctcact gaactcctat
     3901 ctggtactca atattccact gcaaatttat tttacaaagg tttctgtgag ataaaggatt
     3961 tgattgacca atggtgtgtt catgaaaaat ttgtcattag gagaatggcc gttgcaatga
     4021 gtgaaaagtt tgagaaatat tggaaagtgt ctaatattgc actagctgta gcatgcttcc
     4081 ttgaccctag gtacaagaaa atattgattg agttctatat gaaaaaattt catggtgatt
     4141 catacaaagt tcatgtagat gactttgtta gggtcattag aaaattgtat caattctatt
     4201 ctagttgtag tccttcagct ccaaagacaa agacaactac taatgatagt atggatgata
     4261 ccttgatgga aaatgaagat gatgaatttc aaaactattt gcatgagttg aaggattatg
     4321 atcaagtaga gtcaaatgaa ttggataaat atatgtctga accccttttg aagcatagtg
     4381 gtcagtttga tattttatca tggtggaggg gaagggttgc agaatatcct attctcaccc
     4441 aaattgcaag ggatgtgcta gcaatacaag tgtcaactgt tgcttctgag tctgcgttca
     4501 gtgctggtgg tcgtgttgtt gatccttacc gcaatcgtct tggttcggag attgttgaag
     4561 ctttgatatg cacaaaagat tgggtagcag catctagaaa aggtgctaca tattttccaa
     4621 caatgattgg tgatctcgag gtgctagact ctgttattgc tgctgcaaca aatcatgaga
     4681 atcatatgga tgaggatgaa gacgcaatag aattttctaa gaataatgaa gatgtagcaa
     4741 gtggctcctc tccatgagca atgtgtctta tgtttgttga cagatgagcc ttggttgtaa
     4801 tagtttatgc atgctaagtg ctccagatgt gagcaagtga ttatgaatat gtgttttaaa
     4861 ctttatattg tgtcatgtgt gctagtagac ttatatggct tcttatgtta gccaaggggg
     4921 ccccgggatc tggttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct
     4981 acataatacc aacttacact ttacaaaatg ttgtccccca aaatgtagcc attcgtatct
     5041 gctcctaata aaaagaaagt ttcttcacc
//
