LOCUS       sequence-197474-         126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  codes for a fusion of fluorescent protein mGarnet and mito, stains 
            mictochondrial content in live cells, i.e. human cytochrome c 
            oxidase subunit VIII, the mitochondrial targeting sequence mito.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 126)
  AUTHORS   Hense A, Prunsche B, Gao P, Ishitsuka Y, Nienhaus K, Nienhaus GU
  TITLE     Monomeric Garnet, a far-red fluorescent protein for live-cell STED 
            imaging.
  JOURNAL   Sci Rep. 2015 Dec 9;5:18006. doi: 10.1038/srep18006.
  PUBMED    26648024
REFERENCE   2  (bases 1 to 126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-9"; volume: "5:18006. doi"; pages: " 
            10.1038/srep18006"
COMMENT     Sequence Label: mito-mGarnet
FEATURES             Location/Qualifiers
     source          1..126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttggaa ttctgcagat atccatcaca ctggcggccg
      121 ctcgag
//
