LOCUS       sequence-197487-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with 
            mCherry and Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, 
            Cui B
  TITLE     Understanding CRY2 interactions for optical control of intracellular
            signaling.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.
  PUBMED    28916751
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):547. doi"; pages: " 
            10.1038/s41467-017-00648-8"
COMMENT     Sequence Label: CRY2low-mCherry-Raf1
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2438..3145
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2583..2601)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2851..2870
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5158..5175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5164..5388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5434..5862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5521..5540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5731..5752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5871..5891)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5876..6205
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6056..6191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6118..6137
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6272..7066
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6326..6345)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6936..6955
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7240..7361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7277..7296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7331..7350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7410..7426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7410..7426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7423..7445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7434..7450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7458..7464,7465..7482,7483..7488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7503..7524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7641..7658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7812..8397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7892..7911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8568..9359,9360..9428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9191..9210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9429..9533)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatgaagat ggacaaaaag actatagttt ggtttagaag
      961 agacctaagg attgaggata atcctgcatt agcagcagct gctcacgaag gatctgtttt
     1021 tcctgtcttc atttggtgtc ctgaagaaga aggacagttt tatcctggaa gagcttcaag
     1081 atggtggatg aaacaatcac ttgctcactt atctcaatcc ttgaaggctc ttggatctga
     1141 cctcacttta atcaaaaccc acaacacgat ttcagcgatc ttggattgta tccgcgttac
     1201 cggtgctaca aaagtcgtct ttaaccacct ctatgatcct gtttcgttag ttcgggacca
     1261 taccgtaaag gagaagctgg tggaacgtgg gatctctgtg caaagctaca atggagatct
     1321 attgtatgaa ccgtgggaga tatactgcga aaagggcaaa ccttttacga gtttcaattc
     1381 ttactggaag aaatgcttag atatgtcgat tgaatccgtt atgcttcctc ctccttggcg
     1441 gttgatgcca ataactgcag cggctgaagc gatttgggcg tgttcgattg aagaactagg
     1501 gctggagaat gaggccgaga aaccgagcaa tgcgttgtta actagagctt ggtctccagg
     1561 atggagcaat gctgataagt tactaaatga gttcatcgag aagcagttga tagattatgc
     1621 aaagaacagc aagaaagttg ttgggaattc tacttcacta ctttctccgt atctccattt
     1681 cggggaaata agcgtcagac acgttttcca gtgtgcccgg atgaaacaaa ttatatgggc
     1741 aagagataag aacagtgaag gagaagaaag tgcagatctt tttcttaggg gaatcggttt
     1801 aagagagtat tctcggtata tatgtttcaa cttcccgttt actcacgagc aatcgttgtt
     1861 gagtcatctt cggtttttcc cttgggatgc tgatgttgat aagttcaagg cctggagaca
     1921 aggcaggacc ggttatccgt tggtggatgc cggaatgaga gagctttggg ctaccggatg
     1981 gatgcataac agaataagag tgattgtttc aagctttgct gtgaagtttc ttctccttcc
     2041 atggaaatgg ggaatgaagt atttctggga tacacttttg gatgctgatt tggaatgtga
     2101 catccttggc tggcagtata tctctgggag tatccccgat ggccacgagc ttgatcgctt
     2161 ggacaatccc gcgttacaag gcgccaaata tgacccagaa ggtgagtaca taaggcaatg
     2221 gcttcccgag cttgcgagat tgccaactga atggatccat catccatggg acgctccttt
     2281 aaccgtactc aaagcttctg gtgtggaact cggaacaaac tatgcgaaac ccattgtaga
     2341 catcgacaca gctcgtgagc tactagctaa agctatttca agaaccgagg aagacgaatt
     2401 cgatagtgct ggtagtgctg gtagtgctgg tactagtatg gtgagcaagg gcgaggagga
     2461 taacatggcc atcatcaagg agttcatgcg cttcaaggtg catatggagg gctccgtgaa
     2521 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2581 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2641 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2701 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2761 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2821 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2881 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2941 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     3001 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     3061 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     3121 cggcggcatg gacgagctgt acaagtccgg actcagatct cgagtgatgg agcacataca
     3181 gggagcttgg aagacgatca gcaatggttt tggattcaaa gatgccgtgt ttgatggctc
     3241 cagctgcatc tctcctacaa tagttcagca gtttggctat cagcgccggg catcagatga
     3301 tggcaaactc acagatcctt ctaagacaag caacactatc cgtgttttct tgccgaacaa
     3361 gcaaagaaca gtggtcaatg tgcgaaatgg aatgagcttg catgactgcc ttatgaaagc
     3421 actcaaggtg aggggcctgc aaccagagtg ctgtgcagtg ttcagacttc tccacgaaca
     3481 caaaggtaaa aaagcacgct tagattggaa tactgatgct gcgtctttga ttggagaaga
     3541 acttcaagta gatttcctgg atcatgttcc cctcacaaca cacaactttg ctcggaagac
     3601 gttcctgaag cttgccttct gtgacatctg tcagaaattc ctgctcaatg gatttcgatg
     3661 tcagacttgt ggctacaaat ttcatgagca ctgtagcacc aaagtaccta ctatgtgtgt
     3721 ggactggagt aacatcagac aactcttatt gtttccaaat tccactattg gtgatagtgg
     3781 agtcccagca ctaccttctt tgactatgcg tcgtatgcga gagtctgttt ccaggatgcc
     3841 tgttagttct cagcacagat attctacacc tcacgccttc acctttaaca cctccagtcc
     3901 ctcatctgaa ggttccctct cccagaggca gaggtcgaca tccacaccta atgtccacat
     3961 ggtcagcacc accctgcctg tggacagcag gatgattgag gatgcaattc gaagtcacag
     4021 cgaatcagcc tcaccttcag ccctgtccag tagccccaac aatctgagcc caacaggctg
     4081 gtcacagccg aaaacccccg tgccagcaca aagagagcgg gcaccagtat ctgggaccca
     4141 ggagaaaaac aaaattaggc ctcgtggaca gagagattca agctattatt gggaaataga
     4201 agccagtgaa gtgatgctgt ccactcggat tgggtcaggc tcttttggaa ctgtttataa
     4261 gggtaaatgg cacggagatg ttgcagtaaa gatcctaaag gttgtcgacc caaccccaga
     4321 gcaattccag gccttcagga atgaggtggc tgttctgcgc aaaacacggc atgtgaacat
     4381 tctgcttttc atggggtaca tgacaaagga caacctggca attgtgaccc agtggtgcga
     4441 gggcagcagc ctctacaaac acctgcatgt ccaggagacc aagtttcaga tgttccagct
     4501 aattgacatt gcccggcaga cggctcaggg aatggactat ttgcatgcaa agaacatcat
     4561 ccatagagac atgaaatcca acaatatatt tctccatgaa ggcttaacag tgaaaattgg
     4621 agattttggt ttggcaacag taaagtcacg ctggagtggt tctcagcagg ttgaacaacc
     4681 tactggctct gtcctctgga tggccccaga ggtgatccga atgcaggata acaacccatt
     4741 cagtttccag tcggatgtct actcctatgg catcgtattg tatgaactga tgacggggga
     4801 gcttccttat tctcacatca acaaccgaga tcagatcatc ttcatggtgg gccgaggata
     4861 tgcctcccca gatcttagta agctatataa gaactgcccc aaagcaatga agaggctggt
     4921 agctgactgt gtgaagaaag taaaggaaga gaggcctctt tttccccaga tcctgtcttc
     4981 cattgagctg ctccaacact ctctaccgaa gatcaaccgg agcgcttccg agccatcctt
     5041 gcatcgggca gcccacactg aggatatcaa tgcttgcacg ctgaccacgt ccccgaggct
     5101 gcctgtcttc taagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     5161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5341 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5401 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5461 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5521 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5581 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5641 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5701 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5761 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5821 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5881 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5941 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6001 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6121 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6181 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6241 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     6301 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6361 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6421 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6481 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6541 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6601 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6661 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6721 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6781 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6841 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6901 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6961 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7021 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7081 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     7141 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7201 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7261 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7321 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7381 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7441 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7501 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7561 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7621 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7981 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8041 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8101 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8161 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8221 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8281 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8341 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8941 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9421 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9541 ccccgaaaag tgccacctga cgtc
//
