LOCUS       sequence-197489-       10198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus 
            and one at the C-terminus of dCas9; pCDNA3 vector backbone, 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, 
            Farnham PJ, Segal DJ
  TITLE     dCas9-based epigenome editing suggests acquisition of histone 
            methylation is not sufficient for target gene repression.
  JOURNAL   Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 
            10.1093/nar/gkx578.
  PUBMED    28973434
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-09-29- 29"; volume: "45"; issue: 
            "17"; pages: "9901-9916"
COMMENT     Sequence Label: dCas9-FOG1[N+C]
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             702..722
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             741..806
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1032..1058,1059..1061,1062..3545,3546..3551,3552..3617,
                     3618..3620,3621..5135)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1032..1058,1059..1061,1062..3548,3549..3551,
                     3552..5135)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1032..5135
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5349..5396
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5415..5471
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5535..5554)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5579..5627
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5829..6257
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5916..5935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6126..6147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6266..6286)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6271..6600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6451..6586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6513..6532
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6667..7461
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6721..6740)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7331..7350
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7637..7758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7674..7693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7728..7747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7807..7823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7807..7823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7820..7842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7831..7847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7855..7861,7862..7879,7880..7885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7900..7921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8038..8055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8209..8797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8289..8308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8968..9759,9760..9828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9591..9610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9829..9933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10008..10027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat gcccaagaag aagaggaagg
      721 tgggaggctc cggaggaagc gactataagg accacgacgg agactacaag gatcatgata
      781 ttgattacaa agacgatgac gataagggtg gcgggtccgg cggtggatcc ggtaccatgt
      841 ccaggcggaa acagagcaac ccccggcaga tcaagcgttc cctcggagac atggaggcca
      901 gagaggaggt gcagttggtg ggtgccagcc acatggagca aaaggccacg gcacctgaag
      961 ccccgagccc tagtaccgga gggagtggtg gaagcggcgg ttctggtggc tcaggcggca
     1021 gcggccggcc aatggacaag aagtactcca ttgggctcgc tatcggcaca aacagcgtcg
     1081 gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca
     1141 ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg
     1201 agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga
     1261 atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt
     1321 tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac gagcgccacc
     1381 caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca accatatatc
     1441 atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg atctatctcg
     1501 cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac ctgaacccag
     1561 acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat cagcttttcg
     1621 aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc gctaggctgt
     1681 ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag aagaacggcc
     1741 tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa tctaacttcg
     1801 acctggccga agatgccaag cttcaactga gcaaagacac ctacgatgat gatctcgaca
     1861 atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca aagaacctgt
     1921 cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc aaagctccgc
     1981 tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact ttgctgaagg
     2041 cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat cagtctaaaa
     2101 atggctacgc cggatacatt gacggcggag caagccagga ggaattttac aaatttatta
     2161 agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt aacagagaag
     2221 atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag attcacctgg
     2281 gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg aaagataaca
     2341 gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc cccctcgccc
     2401 ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagaccatc actccctgga
     2461 acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa aggatgacta
     2521 actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg ctgtacgagt
     2581 acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc
     2641 cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc aagacgaacc
     2701 ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa tgtttcgact
     2761 ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc cctgggaacg tatcacgatc
     2821 tcctgaaaat cattaaagac aaggacttcc tggacaatga ggagaacgag gacattcttg
     2881 aggacattgt cctcaccctt acgttgtttg aagataggga gatgattgaa gaacgcttga
     2941 aaacttacgc tcatctcttc gacgacaaag tcatgaaaca gctcaagagg cgccgatata
     3001 caggatgggg gcggctgtca agaaaactga tcaatgggat ccgagacaag cagagtggaa
     3061 agacaatcct ggattttctt aagtccgatg gatttgccaa caggaacttc atgcagttga
     3121 tccatgatga ctctctcacc tttaaggagg acatccagaa agcacaagtt tctggccagg
     3181 gggacagtct tcacgagcac atcgctaatc ttgcaggtag cccagctatc aaaaagggaa
     3241 tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg cataagcccg
     3301 agaatatcgt tatcgagatg gcccgagaga accaaactac ccagaaggga cagaagaaca
     3361 gtagggaaag gatgaagagg attgaagagg gtataaaaga actggggtcc caaatcctta
     3421 aggaacaccc agttgaaaac acccagcttc agaatgagaa gctctacctg tactacctgc
     3481 agaacggcag ggacatgtac gtggatcagg aactggacat caatcggctc tccgactacg
     3541 acgtggatgc tatcgtgccc cagtcttttc tcaaagatga ttctattgat aataaagtgt
     3601 tgacaagatc cgataaaaat agagggaaga gtgataacgt cccctcagaa gaagttgtca
     3661 agaaaatgaa aaattattgg cggcagctgc tgaacgccaa actgatcaca caacggaagt
     3721 tcgataatct gactaaggct gaacgaggtg gcctgtctga gttggataaa gccggcttca
     3781 tcaaaaggca gcttgttgag acacgccaga tcaccaagca cgtggcccaa attctcgatt
     3841 cacgcatgaa caccaagtac gatgaaaatg acaaactgat tcgagaggtg aaagttatta
     3901 ctctgaagtc taagctggtc tcagatttca gaaaggactt tcagttttat aaggtgagag
     3961 agatcaacaa ttaccaccat gcgcatgatg cctacctgaa tgcagtggta ggcactgcac
     4021 ttatcaaaaa atatcccaag cttgaatctg aatttgttta cggagactat aaagtgtacg
     4081 atgttaggaa aatgatcgca aagtctgagc aggaaatagg caaggccacc gctaagtact
     4141 tcttttacag caatattatg aattttttca agaccgagat tacactggcc aatggagaga
     4201 ttcggaagcg accacttatc gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg
     4261 gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa
     4321 agaccgaagt acagaccgga ggcttctcca aggaaagtat cctcccgaaa aggaacagcg
     4381 acaagctgat cgcacgcaaa aaagattggg accccaagaa atacggcgga ttcgattctc
     4441 ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac
     4501 tcaaaagcgt caaggaactg ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa
     4561 accccatcga ctttctcgag gcgaaaggat ataaagaggt caaaaaagac ctcatcatta
     4621 agcttcccaa gtactctctc tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg
     4681 cgggcgagct gcagaaaggt aacgagctgg cactgccctc taaatacgtt aatttcttgt
     4741 atctggccag ccactatgaa aagctcaaag ggtctcccga agataatgag cagaagcagc
     4801 tgttcgtgga acaacacaaa cactaccttg atgagatcat cgagcaaata agcgaattct
     4861 ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt gctttctgct tacaataagc
     4921 acagggataa gcccatcagg gagcaggcag aaaacattat ccacttgttt actctgacca
     4981 acttgggcgc gcctgcagcc ttcaagtact tcgacaccac catagacaga aagcggtaca
     5041 cctctacaaa ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg
     5101 aaacaagaat cgacctctct cagctcggtg gagacggtgg tagtggaggt tcaggaggat
     5161 ccggggggag cggagggagc gctagcatgt ccaggcggaa acagagcaac ccccggcaga
     5221 tcaagcgttc cctcggagac atggaggcca gagaggaggt gcagttggtg ggtgccagcc
     5281 acatggagca aaaggccacg gcacctgaag ccccgagccc ttccggtgga ggctccggag
     5341 gtggatctaa aaggccggcg gccacgaaaa aggccggtca ggcaaaaaag aaaaagggtg
     5401 gtagtggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     5461 accctggacc tgcggccgca tgaaagggtt cgatccctac cggttagtaa tgagtttaaa
     5521 cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5581 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     5641 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5701 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5761 agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg gctctagggg
     5821 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5881 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5941 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6001 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6061 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6121 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6181 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6241 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6301 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6361 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6421 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6481 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6541 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6601 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     6661 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6721 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6781 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6841 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6901 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6961 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7021 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7081 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7141 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     7201 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7261 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7321 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7381 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7441 cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac
     7501 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7561 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7621 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7741 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9961 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
    10021 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
    10081 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
    10141 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
