LOCUS       sequence-197563-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Oh JH, Chen SJ, Varshavsky A
  TITLE     A reference-based protein degradation assay without global 
            translation inhibitors.
  JOURNAL   J Biol Chem. 2017 Dec 29;292(52):21457-21465. doi: 
            10.1074/jbc.M117.814236. Epub 2017 Nov 9.
  PUBMED    29122887
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.814236"; journalName: "J Biol Chem"; date: 
            "2017-12-29- 29"; volume: "292"; issue: "52"; pages: "21457-21465"
COMMENT     Sequence Label: pJO629
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     promoter        46..712
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     582..605
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gnnnnnnnnn nnnngggcga ttggagctcc accgcggcga ctttatcatt atcaatactg
       61 ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa
      121 aaaattagcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca
      181 cagaatatat aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata
      241 taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata
      301 ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac
      361 aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga
      421 gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt
      481 ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc
      541 cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa
      601 ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta
      661 gttttaaaac accaagaact tagtttcgaa taaacacaca taaacaaaca aaatcgtacg
      721 ggcctaaaac ataccagatc gccacccgcg ctttaatctg gagaggtgaa gaatacgacc
      781 acctaggcct taagaaccgg taaaacatac cagatcgcca cccgcgcttt aatctggaga
      841 ggtgaagaat acgaccacct accggtcaac aacaacaaca acaacaacaa caactcgagg
      901 cctaaaacat accagatcgc cacccgcgct ttaatctgga gaggtgaaga atacgaccac
      961 ctnnnctcga ganntaggnn ccgncatggc tcnac
//
