LOCUS       sequence-197626-        6784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human MTMR1 PH-PTP domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Bong SM, Son KB, Yang SW, Park JW, Cho JW, Kim KT, Kim H, Kim SJ, 
            Kim YJ, Lee BI
  TITLE     Crystal Structure of Human Myotubularin-Related Protein 1 Provides 
            Insight into the Structural Basis of Substrate Specificity.
  JOURNAL   PLoS One. 2016 Mar 28;11(3):e0152611. doi: 
            10.1371/journal.pone.0152611. eCollection 2016.
  PUBMED    27018598
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-28"; volume: "11(3):e0152611. doi"; pages: " 
            10.1371/journal.pone.0152611. eCollection 2016"
COMMENT     Sequence Label: pHis-hMTMR1
FEATURES             Location/Qualifiers
     source          1..6784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     180..199
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        180..198
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    199..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1808..1831
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(1894..1912)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1908..1955
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1992..2447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2079..2098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2289..2310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2539..3354)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2539..3354)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3262..3281
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3346..3365
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3476..4064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3965..3984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4218..4235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4250..4389
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4315..4334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4475..4497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4491..4682)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5491..6573)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6535..6554
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6574..6651)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
       61 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
      121 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt
      181 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag atttaaatta
      241 actttaagaa ggagatatac atatggaaga ggctccactt ttcccaggag aatcaattaa
      301 agccattgtg aaagatgtca tgtatatctg cccatttatg ggagcagtga gtggaaccct
      361 gacagtgacg gactttaagc tgtacttcaa aaatgtcgag agggacccgc attttatcct
      421 tgatgttccc cttggagtga tcagcagagt ggagaagatt ggagcacaga gccatggaga
      481 caattcctgt ggtatagaga tagtgtgcaa ggatatgagg aacttgcggc ttgcttataa
      541 acaggaagaa cagagtaaac tagggatatt tgaaaacctc aacaaacatg catttcctct
      601 ttctaacgga caggcactat ttgcattcag ctataaagaa aaatttccaa ttaatggctg
      661 gaaagtttat gatccagtat ctgaatataa gagacagggc ttgccaaatg agagttggaa
      721 aatatccaaa ataaacagta attatgagtt ctgtgacacc taccctgcca tcattgttgt
      781 gccaactagt gtaaaagatg atgacctttc aaaagtggca gcttttcgag caaaaggcag
      841 agtccctgtg ttgtcatgga ttcatccgga aagtcaagca acgattaccc gttgcagcca
      901 gccacttgtg ggtcccaatg ataagcgctg caaagaggat gaaaaatact tgcaaacaat
      961 aatggatgct aacgcacagt cacacaagct tatcatcttt gatgctcgac aaaacagtgt
     1021 cgctgatacc aacaagacaa agggtggagg atatgaaagt gaaagtgctt acccaaatgc
     1081 agaacttgtg ttcttggaga tccacaacat tcatgtcatg cgagagtcac tacgcaaatt
     1141 aaaagagatt gtgtaccctt cgatcgatga ggcgcggtgg ctctccaatg tggatgggac
     1201 gcattggctg gaatatataa ggatgctgct tgctggggca gtaagaattg ctgataaaat
     1261 agaatctggg aaaacatctg tggtggtgca ttgcagcgac ggttgggacc gaacagccca
     1321 gctcacatct ctggctatgc taatgttgga cagttactac aggaccatta aaggatttga
     1381 aactctcgta gaaaaggagt ggataagctt tggacacagg tttgcactgc gagtgggcca
     1441 tggtaatgac aaccatgcgg atgctgaccg atctcccata tttctgcagt ttgttgattg
     1501 tgtttggcaa atgacaaggc agtttccttc agcattcgag tttaatgagc tattcttgat
     1561 tacaattttg gatcaccttt atagctgtct ttttgggacc tttttgtgca actgtgaaca
     1621 gcagcgattc aaagaggatg tatatacaaa gacgatatct ttatggtcgt atatcaatag
     1681 ccagctagac gagttttcta atcccttctt tgtgaattat gaaaaccacg tgttatatcc
     1741 tgttgctagt ctgagtcatt tggaattgtg ggtaaattat tatgtacgat ggaatccacg
     1801 gctcgagcac caccaccacc accaccacca ctaattgatt aatacctagg ctgctaaaca
     1861 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     1921 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     1981 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2401 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2521 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     2581 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     2641 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     2701 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     2761 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     2821 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2881 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     2941 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     3001 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     3061 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     3121 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     3181 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     3241 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     3301 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     3361 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     3421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3601 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     4261 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     4321 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     4381 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4441 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4501 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     4561 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     4621 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     4681 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     4741 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     4801 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     4861 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     4921 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     4981 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     5041 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     5101 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     5161 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     5221 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     5281 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     5341 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     5401 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     5461 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5581 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     5641 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     5701 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     5761 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     5821 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     5881 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     5941 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     6001 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     6061 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     6121 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     6181 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     6241 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     6301 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     6361 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     6421 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     6481 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     6541 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     6601 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     6661 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     6721 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     6781 ccac
//
