LOCUS       sequence-197927-        8271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BE1 with C-terminal NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE1
FEATURES             Location/Qualifiers
     source          1..8271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        120..499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        500..703
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     654..674
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     745..764
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        745..763
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             777..1463
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1512..4910,4911..4913,4914..5510,5511,5512..5514,
                     5515..5516,5517..5519,5520..5612)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1512..1535,1536..1538,1539..4025,4026..4028,
                     4029..5612)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5625..5645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5654..5671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5694..5711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5700..5924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5995..6011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5995..6011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6008..6030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6019..6035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6043..6049,6050..6067,6068..6073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6088..6109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6226..6243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6397..6985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6477..6496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7156..7947,7948..8016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7779..7798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8017..8121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8200..8219)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
       61 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      121 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      481 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      541 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      601 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      661 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      721 gatccgctag agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga
      781 gctcagagac tggcccagtg gctgtggacc ccacattgag acggcggatc gagccccatg
      841 agtttgaggt attcttcgat ccgagagagc tccgcaagga gacctgcctg ctttacgaaa
      901 ttaattgggg gggccggcac tccatttggc gacatacatc acagaacact aacaagcacg
      961 tcgaagtcaa cttcatcgag aagttcacga cagaaagata tttctgtccg aacacaaggt
     1021 gcagcattac ctggtttctc agctggagcc catgcggcga atgtagtagg gccatcactg
     1081 aattcctgtc aaggtatccc cacgtcactc tgtttattta catcgcaagg ctgtaccacc
     1141 acgctgaccc ccgcaatcga caaggcctgc gggatttgat ctcttcaggt gtgactatcc
     1201 aaattatgac tgagcaggag tcaggatact gctggagaaa ctttgtgaat tatagcccga
     1261 gtaatgaagc ccactggcct aggtatcccc atctgtgggt acgactgtac gttcttgaac
     1321 tgtactgcat catactgggc ctgcctcctt gtctcaacat tctgagaagg aagcagccac
     1381 agctgacatt ctttaccatc gctcttcagt cttgtcatta ccagcgactg cccccacaca
     1441 ttctctgggc caccgggttg aaaagcggca gcgagactcc cgggacctca gagtccgcca
     1501 cacccgaaag tgataaaaag tattctattg gtttagccat cggcactaat tccgttggat
     1561 gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg ttggggaaca
     1621 cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat agtggcgaaa
     1681 cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc
     1741 gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac gattctttct
     1801 ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa cggcacccca
     1861 tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg atttatcacc
     1921 tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc tacttggctc
     1981 ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta aatccggaca
     2041 actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag ttgtttgaag
     2101 agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta
     2161 aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa aatgggttgt
     2221 tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg aacttcgact
     2281 tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat ctcgacaatc
     2341 tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa aaccttagcg
     2401 atgcaatcct cctatctgac atactgagag ttaatactga gattaccaag gcgccgttat
     2461 ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga cttgacactt ctcaaggccc
     2521 tagtccgtca gcaactgcct gagaaatata aggaaatatt ctttgatcag tcgaaaaacg
     2581 ggtacgcagg ttatattgac ggcggagcga gtcaagagga attctacaag tttatcaaac
     2641 ccatattaga gaagatggat gggacggaag agttgcttgt aaaactcaat cgcgaagatc
     2701 tactgcgaaa gcagcggact ttcgacaacg gtagcattcc acatcaaatc cacttaggcg
     2761 aattgcatgc tatacttaga aggcaggagg atttttatcc gttcctcaaa gacaatcgtg
     2821 aaaagattga gaaaatccta acctttcgca taccttacta tgtgggaccc ctggcccgag
     2881 ggaactctcg gttcgcatgg atgacaagaa agtccgaaga aacgattact ccatggaatt
     2941 ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact
     3001 ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca cagtttactt tacgagtatt
     3061 tcacagtgta caatgaactc acgaaagtta agtatgtcac tgagggcatg cgtaaacccg
     3121 cctttctaag cggagaacag aagaaagcaa tagtagatct gttattcaag accaaccgca
     3181 aagtgacagt taagcaattg aaagaggact actttaagaa aattgaatgc ttcgattctg
     3241 tcgagatctc cggggtagaa gatcgattta atgcgtcact tggtacgtat catgacctcc
     3301 taaagataat taaagataag gacttcctgg ataacgaaga gaatgaagat atcttagaag
     3361 atatagtgtt gactcttacc ctctttgaag atcgggaaat gattgaggaa agactaaaaa
     3421 catacgctca cctgttcgac gataaggtta tgaaacagtt aaagaggcgt cgctatacgg
     3481 gctggggacg attgtcgcgg aaacttatca acgggataag agacaagcaa agtggtaaaa
     3541 ctattctcga ttttctaaag agcgacggct tcgccaatag gaactttatg cagctgatcc
     3601 atgatgactc tttaaccttc aaagaggata tacaaaaggc acaggtttcc ggacaagggg
     3661 actcattgca cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa aagggcatac
     3721 tccagacagt caaagtagtg gatgagctag ttaaggtcat gggacgtcac aaaccggaaa
     3781 acattgtaat cgagatggca cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc
     3841 gagagcggat gaagagaata gaagagggta ttaaagaact gggcagccag atcttaaagg
     3901 agcatcctgt ggaaaatacc caattgcaga acgagaaact ttacctctat tacctacaaa
     3961 atggaaggga catgtatgtt gatcaggaac tggacataaa ccgtttatct gattacgacg
     4021 tcgatgccat tgtaccccaa tcctttttga aggacgattc aatcgacaat aaagtgctta
     4081 cacgctcgga taagaaccga gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga
     4141 aaatgaagaa ctattggcgg cagctcctaa atgcgaaact gataacgcaa agaaagttcg
     4201 ataacttaac taaagctgag aggggtggct tgtctgaact tgacaaggcc ggatttatta
     4261 aacgtcagct cgtggaaacc cgccaaatca caaagcatgt tgcacagata ctagattccc
     4321 gaatgaatac gaaatacgac gagaacgata agctgattcg ggaagtcaaa gtaatcactt
     4381 taaagtcaaa attggtgtcg gacttcagaa aggattttca attctataaa gttagggaga
     4441 taaataacta ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg accgcactca
     4501 ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg
     4561 tccgtaagat gatcgcgaaa agcgaacagg agataggcaa ggctacagcc aaatacttct
     4621 tttattctaa cattatgaat ttctttaaga cggaaatcac tctggcaaac ggagagatac
     4681 gcaaacgacc tttaattgaa accaatgggg agacaggtga aatcgtatgg gataagggcc
     4741 gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata gtaaagaaaa
     4801 ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg aatagtgata
     4861 agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc gatagcccta
     4921 cagttgccta ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc aagaaactga
     4981 agtcagtcaa agaattattg gggataacga ttatggagcg ctcgtctttt gaaaagaacc
     5041 ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc ataattaaac
     5101 taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg gctagcgccg
     5161 gagagcttca aaaggggaac gaactcgcac taccgtctaa atacgtgaat ttcctgtatt
     5221 tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag aagcaacttt
     5281 ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg gaattcagta
     5341 agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac aacaagcaca
     5401 gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact cttaccaacc
     5461 tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa cgatacactt
     5521 ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga ttatatgaaa
     5581 ctcggataga tttgtcacag cttgggggtg actctggtgg ttctcccaag aagaagagga
     5641 aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     5701 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5761 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5821 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5881 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5941 aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6061 cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8161 gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc
     8221 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt a
//
