LOCUS       sequence-198020-        1243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express MBP hnRNPA2 LC with 2 R to K mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, 
            Amaya J, Burke KA, Mittal J, Fawzi NL
  TITLE     Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, 
            Interactions, and Phase Separation Altered by Mutation and Arginine 
            Methylation.
  JOURNAL   Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022.
  PUBMED    29358076
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022."
COMMENT     Sequence Label: MBP-hnRNPA2_LC_R191K_R254K
FEATURES             Location/Qualifiers
     source          1..1243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(560..579)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     gap             1119..1129
                     /estimated_length=11
     gap             1140..1154
                     /estimated_length=15
ORIGIN
        1 cnnntctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 tagtaacggc tgcggccacc atagccgcca gaaccaccgc taccacccgg gccgtagtta
      121 ccgccaccat acggaccacc catattacgg gaaccgccaa aattgccgga tttcatcggg
      181 ccgtaattgc tcggttgctg gttgtagttg ccaaaatcat tgtaattgcc gctaccatag
      241 ttaccaccgc catagttatc gtagccacca ccataaccac caccctggtt accgtagcct
      301 ggaccgccac cgccataacc gcctttacca ccgccgtaac ccgggctacc accgaaattg
      361 ccgccgcccg gaccaccacc gtaaccatta tagccatcgc caaagccgcg accgctaccg
      421 taaccgtcgg aaccaccacg gaaattgctg cccggacccg gaccgaagtt gccgccacca
      481 ccgcggctat caccgaaacc aaagttgcca cctttaccca tataattcag ggtggtgaat
      541 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
      601 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      661 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      721 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      781 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      841 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      901 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgn
      961 nctaatgttc cggcgttatt tcttgatnnn tctgacnann nccnatcaac agtattattt
     1021 nnnccnatga aganggaccn cnactgggcn nggaacatcn ggnccnattg gnnnnnnnna
     1081 annngnctgn angngggccc ttaagttnnn nnnggcngnn nnnnnnnnng ngnnnnnaan
     1141 nnnnnnnnnn nnnnaattcc cnnnnnngna annnnnngnn nngggggnnn ggcngnttnn
     1201 nnnncnnnaa nggnannnnn nncntncccn nnnnngngnn ncc
//
