LOCUS       sequence-198167-        1956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LI resistance gene module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wu D, Schandry N, Lahaye T
  TITLE     A modular toolbox for Golden-Gate-based plasmid assembly streamlines
            generation of Ralstonia solanacearum species complex knockout 
            strains and multi-cassette complementation constructs.
  JOURNAL   Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632.
  PUBMED    29077245
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632."
COMMENT     Sequence Label: LI F-G Tet
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..651)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             757..1956
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
       61 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
      121 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
      181 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
      241 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
      301 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
      361 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
      421 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
      481 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
      541 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
      601 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
      661 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
      721 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
      781 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
      841 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
      901 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
      961 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc
     1021 atcatggcga cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     1081 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     1141 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     1201 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     1261 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     1321 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     1381 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     1441 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     1501 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     1561 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     1621 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     1681 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     1741 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     1801 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     1861 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     1921 ctttggagcg gcgcagggca acgagccgat cgctga
//
