LOCUS       sequence-19820-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Soriano P
  TITLE     The PDGF alpha receptor is required for neural crest cell 
            development and for normal patterning of the somites.
  JOURNAL   Development. 1997 Jul . 124(14):2691-700.
  PUBMED    9226440
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1997 Jul . 124(14):2691-700."
COMMENT     Sequence Label: PGKneolox2DTA
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..54
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        84..583
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(119..142)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     516..535
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gtggatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
       61 cctcgacctg caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
      121 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      181 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      241 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      301 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      361 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      421 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      481 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      541 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatctgg
      601 gatcgtccat agaataagat ggattgcacg caagttctcc tgccgcatga ctggagaggc
      661 tactcggcta tg
//
