LOCUS       sequence-198302-        3210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing HOXC5 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HOXC5
FEATURES             Location/Qualifiers
     source          1..3210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(767..866)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(883..902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(884..902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1025..1044)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1036..1845
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1103..1122)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1992..2580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2481..2500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2734..2751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2910..2937)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3029..3115)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3164..3186
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3178..3195
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3179..3195
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgagctccta cgtagccaat
      121 tcattctata agcagagccc caatatccct gcctataaca tgcaaacttg tgggaactat
      181 ggatcggcct cagaggtgca ggcatccagg tactgctacg gcggattgga cttaagcatc
      241 actttcccac cgcctgcgcc ttccaactct ctacacgggg tagacatggc tgcaaacccc
      301 agagctcatc ctgacagacc agcatgtagt gccgcagcag ctccgggaca tgctccgggt
      361 agagatgaag cggctccttt gaacccaggg atgtacagtc aaaaggcggc tcgaccagca
      421 ctggaggagc gagctaagag cagtggggag attaaagagg agcaggcaca gacagggcag
      481 cctgccggat taagtcagcc accagccccg ccacagattt acccgtggat gaccaaactg
      541 cacatgagcc acgagacgga cggcaagcga tcccgaacca gttacacgcg ctaccagact
      601 ctggaactcg agaaagaatt ccactttaac cgctacctca ctcgccgcag gcgcatagag
      661 atcgccaaca acttgtgtct caatgagaga cagatcaaga tctggttcca gaaccgcagg
      721 atgaagtgga agaaagattc caaaatgaaa agcaaagagg ctctttaccc aactttcttg
      781 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      841 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
      901 tacatggtca tagctgtttc ttggcagctc tggcccgtgt ctcaaaatct ctgatgttac
      961 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1021 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1081 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1141 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1201 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1261 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1321 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1381 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1441 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1501 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1561 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     1621 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1681 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1741 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1801 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     1861 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     1921 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     1981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     2881 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     2941 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3001 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3061 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3121 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3181 aaaacgacgg ccagtcttaa gctcgggccc
//
