LOCUS       sequence-198396-        8451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of c-Jun.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Song W, Ma Y, Wang J, Brantley-Sieders D, Chen J
  TITLE     JNK signaling mediates EPHA2-dependent tumor cell proliferation, 
            motility, and cancer stem cell-like properties in non-small cell 
            lung cancer.
  JOURNAL   Cancer Res. 2014 May 1;74(9):2444-54. doi: 
            10.1158/0008-5472.CAN-13-2136. Epub 2014 Mar 7.
  PUBMED    24607842
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-13-2136"; journalName: "Cancer Res"; date: 
            "2014-05-1- 1"; volume: "74"; issue: "9"; pages: "2444-54"
COMMENT     Sequence Label: pCLXSN-c-JUN
FEATURES             Location/Qualifiers
     source          1..8451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(572..592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        577..906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      757..892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             966..1760
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1020..1039)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1630..1649
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1820..2413
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          2758..2823
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2953..2973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3245..3379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3282..3301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3336..3355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3837..3971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4036..4053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4207..4795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4287..4306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4966..5757,5758..5826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5589..5608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5827..5931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5999..6017
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     6177..6196
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6341..6720
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6721..6924
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6875..6895
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6925..7100
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    7163..7362
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7563..7979
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7909..7931
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7947..7963
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             complement(8028..8039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctcggtggc agggggcagc ggcagcggcg gcttcagcgc cagcctgcac agcgagccgc
       61 cggtctacgc aaacctcagc aacttcaacc caggcgcgct gagcagcggc ggcggggcgc
      121 cctcctacgg cgcggccggc ctggcctttc ccgcgcaacc ccagcagcag cagcagccgc
      181 cgcaccacct gccccagcag atgcccgtgc agcacccgcg gctgcaggcc ctgaaggagg
      241 agcctcagac agtgcccgag atgcccggcg agacaccgcc cctgtccccc atcgacatgg
      301 agtcccagga gcggatcaag gcggagagga agcgcatgag gaaccgcatc gctgcctcca
      361 agtgccgaaa aaggaagctg gagagaatcg cccggctgga ggaaaaagtg aaaaccttga
      421 aagctcagaa ctcggagctg gcgtccacgg ccaacatgct cagggaacag gtggcacagc
      481 ttaaacagaa agtcatgaac cacgttaaca gtgggtgcca actcatgcta acgcagcagt
      541 tgcaaacatt ttgactcgag gatccggctg tggaatgtgt gtcagttagg gtgtggaaag
      601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      661 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      721 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      841 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      901 tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca agagacagga tgaggatcgt
      961 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1021 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1081 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1141 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1201 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1261 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1321 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1381 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1441 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1501 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     1561 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     1621 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     1681 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     1741 ttcttgacga gttcttctga gcgggactct ggggttcgat aaaataaaag attttattta
     1801 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     1861 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     1921 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     1981 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     2041 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     2101 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     2161 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     2221 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     2281 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     2341 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     2401 ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac
     2461 ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     2521 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2581 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     2641 cacgtagcga tagcggagtg tatctagcta ggtagctaga ggatctttgt gaaggaacct
     2701 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     2761 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     2821 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     2881 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     2941 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     3001 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     3061 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg
     3121 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3181 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3241 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3301 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3361 atcttatcat gtctggatca gcttcagaag atggcggagg gcctccaaca cagtaatttt
     3421 cctcccgact cttaaaatag aaaatgtcaa gtcagttaag caggaagtga ctaactgacc
     3481 gcagctggcc gtgcgacatc ctcttttaat tagttgctag gcaactgccc tccagagggc
     3541 agtgtggttt tgcaagagga agcaaaaagc ctctccaccc aggcctagaa tgtttccacc
     3601 caatcattac tatgacaaca gctgtttttt ttagtattaa gcagaggccg ggggcccctg
     3661 gcctccgctt actctggaga aaaagaagag aggcattgta gaggcttcca gaggcaactt
     3721 gtcaaaacag gactgcttct atttctgtca cactgtctgg ccctgtcaca aggtccagca
     3781 cctccatacc ccctttaata agcagtttgg gaacgggtgc gggtcttact ccgcccatcc
     3841 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3901 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3961 tttttggagg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4621 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4861 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4921 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4981 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5041 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5101 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5161 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5221 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5281 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5341 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5401 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5461 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5521 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5581 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5641 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5701 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5761 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5821 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5881 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5941 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6001 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     6061 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     6121 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     6181 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6241 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     6301 gcgatcggtg cgggcctctt cgctattacg ccagcgcgtt gacattgatt attgactagt
     6361 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6421 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6481 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6541 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6601 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6661 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6721 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     6781 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6841 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     6901 tgggaggtct atataagcag agctcaataa aagagcccac aacccctcac tcggcgcgcc
     6961 agtcttccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
     7021 tgcatccgaa tcgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     7081 cacgacgggg gtctttcatt tgggggctcg tccgggattt ggagacccct gcccagggac
     7141 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
     7201 tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta gttagctaac tagctctgta
     7261 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
     7321 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
     7381 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
     7441 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
     7501 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
     7561 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     7621 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     7681 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     7741 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     7801 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     7861 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     7921 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
     7981 gaattgcggc cgcaattcgt taactcgaga tgactgcaaa gatggaaacg accttctatg
     8041 acgatgccct caacgcctcg ttcctcccgt ccgagagcgg accttatggc tacagtaacc
     8101 ccaagatcct gaaacagagc atgaccctga acctggccga cccagtgggg agcctgaagc
     8161 cgcacctccg cgccaagaac tcggacctcc tcacctcgcc cgacgtgggg ctgctcaagc
     8221 tggcgtcgcc cgagctggag cgcctgataa tccagtccag caacgggcac atcaccacca
     8281 cgccgacccc cacccagttc ctgtgcccca agaacgtgac agatgagcag gagggcttcg
     8341 ccgagggctt cgtgcgcgcc ctggccgaac tgcacagcca gaacacgctg cccagcgtca
     8401 cgtcggcggc gcagccggtc aacggggcag gcatggtggc tcccgcggta g
//
