LOCUS       sequence-198600-        8641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8641)
  TITLE     pAM-PAT-ProUBQ10 GW
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAM-PAT-35S-GW-Luc
FEATURES             Location/Qualifiers
     source          1..8641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(458..637)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1106..1450
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1394..1414
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     1452..1468
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1453..1469
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1496..1620
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1645..1650,1651..1668,1669..1675)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1729..2388
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1795..1814)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2730..3035
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2914..2933
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3076..3200)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3211..4863
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3277..3295)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(4876..4892)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4876..4892)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    5175..5199
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5311..6020
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6093..6184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6185..6253,6254..7045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6403..6422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7216..7804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7705..7724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8116..8140
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8234..8486)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(8506..8641,1..416))
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg
       61 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc
      121 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt
      181 gccttccagg gacccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc
      241 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc
      301 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc
      361 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatggta
      421 gatcccctcg atcgagttga gagtgaatat gagactctaa ttggataccg aggggaattt
      481 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
      541 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
      601 aacccgcggc tcagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
      661 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc
      721 agattgtcgt ttcccgcctt cggtttgggc gcgcccggtt cagaagacca gagggctatt
      781 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
      841 tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg ccatcattgc
      901 gataaaggaa aggctatcgt tcaagatgcc tctaccgaca gtggtcccaa agatggaccc
      961 ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     1021 gattgatgtg atacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     1081 cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     1141 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     1201 atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta
     1261 ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg
     1321 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     1381 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1441 tggagaggac ctcgaggtcg acggtatcga taagcttggt accgaattcc ccatcacaag
     1501 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     1561 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     1621 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     1681 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1741 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1801 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1861 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1921 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     1981 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2041 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2101 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2161 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2221 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2281 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     2341 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     2401 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2461 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     2521 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     2581 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     2641 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     2701 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     2761 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     2821 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     2881 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     2941 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3001 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3061 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3121 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3181 gctttcttgt acaaagtggt tgcgatcgcc atggaagacg ccaaaaacat aaagaaaggc
     3241 ccggcgccat tctatcctct tgaggatgga accgctggag agcaactgca taaggctatg
     3301 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac
     3361 atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     3421 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     3481 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     3541 gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa
     3601 aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt
     3661 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     3721 catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa
     3781 acaattgcac tgataatgaa ctcctctgga tctactgggt tacctaaggg tgtggccctt
     3841 ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa
     3901 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     3961 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     4021 gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc
     4081 ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     4141 gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc
     4201 ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg
     4261 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     4321 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta
     4381 tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     4441 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     4501 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
     4561 cccgctgaat tggaatcaat attgttacaa caccccaaca tcttcgacgc gggcgtggca
     4621 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     4681 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     4741 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     4801 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
     4861 taagagctcc cgggggatcc actagttcta gagtccgcaa aaatcaccag tctctctcta
     4921 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     4981 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     5041 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     5101 gaccgggcgg ccgccaccgc ggtggagggg gatcagattg tcgtttcccg ccttcagttt
     5161 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     5221 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     5281 gtacatcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
     5341 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
     5401 agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     5461 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     5521 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     5581 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     5641 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     5701 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     5761 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     5821 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     5881 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     5941 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
     6001 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
     6061 gggatgggcg atcagcccga aagctacccc tatttgttta tttttctaaa tacattcaaa
     6121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7861 catggactct agctagagga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     7921 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     7981 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     8041 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     8101 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     8161 taataacaca ttgcggacgt ttttaatgat cgaatactaa cgtctctacc agatatcagc
     8221 ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     8281 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     8341 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     8401 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     8461 ctttattgcc aaatgtttga acgatctgct tgactctagg ggtcatcaga tttcggtgac
     8521 gggcaggacc ggacggggcg gcaccggcag gctgaagtcc agctgccaga aacccacgtc
     8581 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
     8641 c
//
