LOCUS       sequence-198665-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human Invariant chain with a CLIP replacement with in frame 
            restriction sites (KpnI/XhoI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Mensali N, Grenov A, Pati NB, Dillard P, Myhre MR, Gaudernack G, 
            Kvalheim G, Inderberg EM, Bakke O, Walchli S
  TITLE     Antigen-delivery through invariant chain (CD74) boosts CD8 and CD4 T
            cell immunity.
  JOURNAL   Oncoimmunology. 2019 Jan 11;8(3):1558663. doi: 
            10.1080/2162402X.2018.1558663. eCollection 2019.
  PUBMED    30723591
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Oncoimmunology."; date: "2019-01-11"; volume: "8(3):1558663. doi"; 
            pages: " 10.1080/2162402X.2018.1558663. eCollection 2019"
COMMENT     Sequence Label: pENTR-li-p33_RS-CLIP
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(744..843)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(860..879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(861..879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(884..900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(884..900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcatatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
      121 ggatgaccag cgcgacctta tctccaacaa tgagcaactg cccatgctgg gccggcgccc
      181 tggggccccg gagagcaagt gcagccgcgg agccctgtac acaggctttt ccatcctggt
      241 gactctgctc ctcgctggcc aggccaccac cgcctacttc ctgtaccagc agcagggccg
      301 gctggacaaa ctgacagtca cctcccagaa cctgcagctg gagaacctgc gcatgaagct
      361 tcccaagcct cccaagcctg tgagcaaggg taccgcagca ctcgagcaga atgccaccaa
      421 gtatggcaac atgacagagg accatgtgat gcacctgctc cagaatgctg accccctgaa
      481 ggtgtacccg ccactgaagg ggagcttccc ggagaacctg agacacctta agaacaccat
      541 ggagaccata gactggaagg tctttgagag ctggatgcac cattggctcc tgtttgaaat
      601 gagcaggcac tccttggagc aaaagcccac tgacgctcca ccgaaagagt cactggaact
      661 ggaggacccg tcttctgggc tgggtgtgac caagcaggat ctgggcccag tccccatgtg
      721 aggatccaag ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa
      781 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
      841 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
      901 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacagataa aatatatcat
      961 catgaacata
//
