LOCUS       sequence-198675-        1351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HLA-B150101 cloned as a His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1351)
  TITLE     HLA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR-HLA-B15-His
FEATURES             Location/Qualifiers
     source          1..1351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1194..1211
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1237..1336)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
       61 tgccaacttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg ctggtcatgg
      121 cgccccgaac cgtcctcctg ctgctctcgg gagccctggc cctgaccgag acctgggccg
      181 gctcccactc catgaggtat ttctacaccg ccatgtcccg gcccggccgc ggggagcccc
      241 gcttcatcgc agtgggctac gtggacgaca cccagttcgt gaggttcgac agcgacgccg
      301 cgagtccgag gatggcgccc cgggcgccat ggatagagca ggaggggccg gagtattggg
      361 accgggagac acagatctcc aagaccaaca cacagactta ccgagagagc ctgcggaacc
      421 tgcgcggcta ctacaaccag agcgaggccg ggtctcacac cctccagagg atgtacggct
      481 gcgacgtggg gccggacggg cgcctcctcc gcgggcatga ccagtccgcc tacgacggca
      541 aggattacat cgccctgaac gaggacctga gctcctggac cgcggcggac acggcggctc
      601 agatcaccca gcgcaagtgg gaggcggccc gtgaggcgga gcagtggaga gcctacctgg
      661 agggcctgtg cgtggagtgg ctccgcagat acctggagaa cgggaaggag acgctgcagc
      721 gcgcggaccc cccaaagaca catgtgaccc accaccccat ctctgaccat gaggccaccc
      781 tgaggtgctg ggccctgggc ttctaccctg cggagatcac actgacctgg cagcgggatg
      841 gcgaggacca aactcaggac accgagcttg tggagaccag accagcagga gatagaacct
      901 tccagaagtg ggcagctgtg gtggtgcctt ctggagaaga gcagagatac acatgccatg
      961 tacagcatga ggggctgccg aagcccctca ccctgagatg ggagccatct tcccagtcca
     1021 ccatccccat cgtgggcatt gttgctggcc tggctgtcct agcagttgtg gtcatcggag
     1081 ctgtggtcgc tactgtgatg tgtaggagga agagctcagg tggaaaagga gggagctact
     1141 ctcaggctgc gtccagcgac agtgcccagg gctctgatgt gtctctcaca gctcatcacc
     1201 atcaccatca ttgactcgag aagggtgggc gcgccgaccc agctttcttg tacaaagttg
     1261 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1321 ataaaatcat tattgccatc cagctgattc c
//
