LOCUS       sequence-198768-       11716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synOptopatch construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF43: Cre-on CAG-CheRiff-CFP (DIO)
FEATURES             Location/Qualifiers
     source          1..11716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2663..2966
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2968..3245
                     /label=chicken beta-actin promoter
     intron          3247..4264
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4188..4210
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4272..4291
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4353..4386
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4449..4482
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(4496..5209,5210..5212))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4541..4562)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4890..4909
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5149..5170
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5282..6229)
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     regulatory      6226..6235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6254..6287)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6334..6367)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6408..6996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6461..6481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6879..6890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6999..7015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7000..7016)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7521..7701
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7727..7744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7733..7957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8003..8431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8090..8109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8300..8321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8440..8460)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8445..8774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8625..8760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8687..8706
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8822..8869
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8888..9262
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9392..9513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9429..9448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9483..9502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9562..9578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9562..9578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9575..9597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9586..9602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9610..9616,9617..9634,9635..9640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9655..9676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9793..9810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9964..10552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10044..10063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10723..11514,11515..11583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11346..11365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11584..11688)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta actagttatt aatagtaatc aattacgggg tcattagttc
     2641 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2701 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2761 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2821 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2881 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2941 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     3001 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     3061 cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     3121 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     3181 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     3241 gggcggggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     3301 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     3361 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     3421 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     3481 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     3541 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     3601 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     3661 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     3721 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     3781 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     3841 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     3901 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     3961 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     4021 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     4081 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     4141 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     4201 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     4261 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgat
     4321 ttgataccgc gggcccggga tctcgaggat ccataacttc gtataaagta tcctatacga
     4381 agttatatca aaataggaag accaatgctt caccatcgac ccgaattgcc aagcatcacc
     4441 atcgacccat aacttcgtat aatgtatgct atacgaagtt atactagcta gctaattact
     4501 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4561 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     4621 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4681 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gtgggccttg atgccgttct
     4741 tctgcttgtc ggcggtgata tagacgttgt ggctgatgta gttgtactcc agcttgtgcc
     4801 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4861 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4921 tggtacgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4981 tgtggtcggg gtagcggctg aagcactgca cgccccaggt cagggtggtc acgagggtgg
     5041 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     5101 tggcatcgcc ctcgccctcg ccggacacgc tgaacctgtg gccgtttacg tcgccgtcca
     5161 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accacgttga
     5221 tgtcgatctg gtccaggggg atgtactcgc cctcgctggt gatcctgctc ttggcggccg
     5281 ccactttatc ttcctctgtc acgaaggcca gcacgtccac cttgcgtccc agaaactggg
     5341 agctcactgg tctcctgata tcgccgtaca taatgatatg ctcgtgaatt ttgatccgca
     5401 ggaagtgccc cagcagtccc caaatattct tggacagcag gtcggcgatg gtatggccaa
     5461 tagtggaccc agccacgctc agggtgtgca tgccttcagg cccaaagatg aacaggccgg
     5521 gaaacatcag ccaactagaa tagtacacgg cagtcatggc cagcaccagt ttcttacagc
     5581 cgccggcagg catgatatag taagactcca cgtagatgat tccagcgttg tagaatgtct
     5641 gggttccata caccaggccg atacagtaaa acagccactt cacccaccca gtggccagag
     5701 cggctgtcac tcccatgcaa atagttccca ggtcggacac cagcagagcc attgtccgct
     5761 tattgtatgc ctcactcagg ccggtgatgt tagacagatg aatcaggatc acgggacatg
     5821 tcagcagcca ttcggcatat ctcagccatg gggtaatgtt ccctccgtac aggaacagca
     5881 tagcaggact ggtgaactcg aaatagatct ccagaatcac tttgatcagc tccacggagc
     5941 acacgtacac ttcctcccat cccacgctgg ccttataggc gtgccagccg tagaaagcca
     6001 gaaacagggc agacaggaac acggcaaacc actggaagcc cctagctgtt tcctgctccc
     6061 acagggcccc atagtagatg tagatggagc tgcactgttc ctcataccca tgcacggggt
     6121 ggtagggtgg attcacttga tatccagctg gggcatggta ctcactgcct ccggcagaat
     6181 cgtttccagg tggggcgctg tgagcgtctg gagcaggagc tccgcccatg gtggcggcct
     6241 aggcgcgcct agtataactt cgtataggat actttatacg aagttatcat tgggattctt
     6301 cctattttga tccaagcatc accatcgacc cccataactt cgtatagcat acattatacg
     6361 aagttattcc ctcgaagagg ttcgaattcg atatcaagct tatcgataat caacctctgg
     6421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6541 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6601 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6661 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6721 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6781 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6841 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6901 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6961 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     7021 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     7081 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     7141 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7201 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     7261 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     7321 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     7381 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     7441 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     7501 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7561 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7621 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7681 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     7741 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7801 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7861 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7921 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7981 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8041 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8101 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8161 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8221 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8281 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8341 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8401 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     8461 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8521 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8581 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8641 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8701 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8761 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     8821 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     8881 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     8941 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     9001 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     9061 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     9121 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     9181 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     9241 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     9301 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     9361 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     9421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9481 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     9541 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11701 aaaagtgcca cctgac
//
