LOCUS       sequence-198790-       12584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl 
            transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in 
            piggyBac (PB) transposon vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Khoueiry R, Sohni A, Thienpont B, Luo X, Velde JV, Bartoccetti M, 
            Boeckx B, Zwijsen A, Rao A, Lambrechts D, Koh KP
  TITLE     Lineage-specific functions of TET1 in the postimplantation mouse 
            embryo.
  JOURNAL   Nat Genet. 2017 Jul;49(7):1061-1072. doi: 10.1038/ng.3868. Epub 2017
            May 15.
  PUBMED    28504700
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3868"; 
            journalName: "Nat Genet"; date: "2017-07"; volume: "49"; issue: "7";
            pages: "1061-1072"
COMMENT     Sequence Label: pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV
FEATURES             Location/Qualifiers
     source          1..12584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(1075..1093,1094..1116,1117..1135,1136..1158,1159..1177,
                     1178..1200,1201..1219,1220..1242,1243..1261,1262..1284,
                     1285..1303,1304..1326,1327..1345)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1378..1416
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1413..1437
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1901..1910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1913..1939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(8001..8575,8576..8578,8579..8587)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8168..8185)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8395..8414
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8588..8590,8591..8593,8594..9307)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8633..8654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8894..8913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9241..9262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9692..9709)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9698..9922
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9969..9985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9993..10009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10017..10023,10024..10041,10042..10047))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10062..10083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   10310..10372
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10396..10414)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10435..10451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10459..10475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10483..10489,10490..10507,10508..10513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10528..10549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10666..10683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10837..11425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10917..10936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11596..12387,12388..12456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12219..12238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12457..12561)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgacaacg gcaagcttac atcgagttta ccactcccta
     1081 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
     1381 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1441 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1501 cgcgggactc agacacccgg gatcctgaga acttcagggt gagtttgggg acccttgatt
     1561 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     1621 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     1681 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     1741 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     1801 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     1861 tatattgtac ttcagcacag ttttagagaa caattgatcc gccaccatgg gttatccata
     1921 tgatgttcca gattatgctt ctcggtcccg ccccgcaaag ccttccaaat cagtcaaaac
     1981 aaagctacag aaaaagaaag acatccagat gaagacgaag acatcaaagc aggctgtcag
     2041 acatggggca tcagcgaagg cagtgaaccc cggaaaaccg aagcaattaa ttaaaagaag
     2101 agatggtaag aaggaaacgg aagacaaaac cccgacaccg gcgccgagtt ttctgacgcg
     2161 agccggggcc gcccgaatga atcgggatcg aaaccaagtt ctttttcaga acccagactc
     2221 cttaacttgc aacgggttta caatggctct tcgaagaacc tctcttagct ggcgactctc
     2281 ccagcgccca gtagtcacac ccaaacctaa gaaagtacca ccttcaaaga agcagtgtac
     2341 acataatatc caggacgagc cgggggtaaa gcactctgaa aatgattcag tccccagcca
     2401 acacgctacc gtgtccccag gcacagagaa tggtgaacag aacaggtgtt tagttgaagg
     2461 ggagagccaa gagataaccc agtcttgccc tgtatttgag gaaagaatag aagataccca
     2521 aagctgtatt tccgcatctg ggaacctgga agcagaaatt tcttggccac tggaagggac
     2581 acattgtgaa gaactacttt ctcatcagac atctgataat gagtgcactt cccctcaaga
     2641 gtgtgctcca ttgccccaaa gatcaacttc cgaagttacc tctcagaaaa acactagcaa
     2701 tcagttagca gacttgagct cacaagtaga gtctatcaag ctgtctgatc cttctccgaa
     2761 tcctacggga agcgaccata atggctttcc agactccagt tttaggatcg ttcctgaatt
     2821 ggacctgaaa acctgcatgc ctcttgatga gtctgtgtat cccacagctc tgataagatt
     2881 catcttggca ggctcacagc cggatgtctt tgatactaag ccccaagaaa aaacccttat
     2941 aacaacccca gaacaagtgg gaagccaccc aaatcaagtt ctggatgcca cctctgtcct
     3001 aggacaggcc tttagtactc ttccacttca gtggggattt tctggtgcta acttagttca
     3061 agttgaggcc ctgggcaagg gttcagactc accagaggat cttggtgcta ttactatgct
     3121 aaaccaacaa gaaactgtgg ctatggatat ggataggaat gctaccccgg acctgcctat
     3181 cttccttcct aagcctccaa acacagttgc tacctacagt agccctcttt tagggcctga
     3241 gccccacagc tctacctcct gtgggcttga agtccaaggt gctaccccga ttttaacttt
     3301 ggactcagga cacactcccc aactcccacc aaaccctgag tcaagttcag tacctctggt
     3361 aatagctgca aacggtacgc gagctgagaa gcagtttggt accagccttt tcccagccgt
     3421 cccacaaggg tttacagtcg cagccgagaa tgaagtccag catgccccat tagatcttac
     3481 ccagggttct caggctgctc ccagcaaact ggaaggagag atttctcggg tcagcatcac
     3541 tggctcagct gatgtcaaag ccacagcgat gtccatgccg gttacacagg ccagtacctc
     3601 ttctccccca tgtaattcta cgccgccaat ggtggagagg aggaagcgca aggcctgtgg
     3661 ggtctgtgag ccgtgccagc agaaggccaa ctgtggggag tgcacctact gcaagaatcg
     3721 aaagaacagc caccagatct gtaagaagag gaaatgcgag gtgctcaaaa agaagccaga
     3781 ggccacctca caggcacagg ttacaaaaga aaacaagagg ccccagaggg aaaagaagcc
     3841 caaagtttta aagacagact ttaacaacaa accagtaaat ggccccaaat cagagtccat
     3901 ggactgcagt agacgtggtc atggggaaga agagcaaagg ttggatttga tcacacatcc
     3961 ccttgaaaat gtaaggaaga atgcaggcgg catgacggga attgaggtgg agaagtgggc
     4021 gccaaacaag aaatcacact tagcagaggg tcaagtcaaa ggaagctgtg atgccaattt
     4081 aactggggtg gaaaaccccc agccctctga agatgacaag cagcaaacca acccttctcc
     4141 gacatttgcc cagaccataa ggaacggcat gaaaaatgta cactgtttac ccactgacac
     4201 acaccttccg ctcaacaaat tgaatcatga ggaatttagc aaggcgttgg gaaataattc
     4261 ctctaaattg ctgactgatc cctcaaactg caaggatgcc atgagtgtca ccacctccgg
     4321 gggggagtgt gatcatctca aggggccaag gaacaccctg ctcttccaga agcctggctt
     4381 gaactgcagg tctggtgcag aacccaccat ctttaataat caccctaata cacacagcgc
     4441 tgggagtcga cctcaccctc ctgagaaggt acccaacaaa gaacccaaag atggctctcc
     4501 ggttcagcca agtcttctat ccctaatgaa ggacaggaga ttaacgctgg aacaagtggt
     4561 agccatagaa gccctgactc agctctcaga agcgccctca gagagctcct ccccatccaa
     4621 gccggagaag gatgaagaag cgcatcagaa aacggctagt ctgcttaaca gttgcaaggc
     4681 tatccttcat tcggtgagaa aagacctcca agacccaaac gttcaaggaa agggccttca
     4741 ccatgacact gttgtcttta atggccagaa cagaacattc aagtcacctg atagtttcgc
     4801 tactaaccaa gcacttataa aatcacaagg atatccaagt tcacctacag ctgagaagaa
     4861 aggcgctgct ggtgggagag ctccctttga tggttttgag aattcacatc cattgcccat
     4921 agaaagtcac aatcttgaaa actgcagtca ggtactaagc tgcgatcaga atttgagttc
     4981 ccatgaccca tcatgccagg atgcacccta cagtcaaatt gaagaagatg tcgcagcaca
     5041 attgacccaa cttgcatcca cgattaatca catcaacgcc gaggtcagga atgctgaaag
     5101 tacaccagaa agccttgttg caaagaacac aaagcaaaaa cacagtcagg agaaacgcat
     5161 ggtacatcag aagccaccct ctagtacaca aactaaaccc agtgtaccat cagcaaagcc
     5221 aaagaaggcc cagaaaaagg caagagcgac cccacacgca aacaagcgga aaaagaaacc
     5281 cccagcccga agctctcaag aaaatgatca gaagaagcaa gagcagctag ctatagagta
     5341 tagtaaaatg catgacattt ggatgtcatc taagtttcaa aggtttggcc agtctagtcc
     5401 acgtagtttt cctgttctgt tgagaaatat tcctgtcttt aaccaaatat taaagcccgt
     5461 gactcagagt aaaacaccct cacaacataa tgagttattt cctcctatca atcagataaa
     5521 attcacaaga aaccctgaat tggcaaagga gaaagtgaag gttgaaccat cagattctct
     5581 gccaacatgc caattcaaga cagaatccgg tgggcagaca tttgcagagc cggcagataa
     5641 ttctcaggga cagcccatgg tgagtgtcaa tcaggaagct caccctctgc cccagtcccc
     5701 accatctaac cagtgtgcta atataatggc tggtgctgcc caaacacagt ttcatctagg
     5761 cgctcaagag aacctggtgc atcagatacc accaccgacg ttgcctggta cctcccctga
     5821 cacactcttg ccagacccag catcaatcct caggaaaggg aaagtgttac atttcgatgg
     5881 aatcacggtg gtcacagaga agcgtgaagc tcaaacatca agcaatggac cactgggtcc
     5941 caccacagac agtgcacagt cagaatttaa agagagcatc atggacttac ttagcaagcc
     6001 tgcaaaaaac ctgatagctg gattgaagga acaggaagct gcaccctgtg actgtgatgg
     6061 aggtacacaa aaagaaaaag gcccatatta tacacacctt ggggcaggac caagtgtggc
     6121 tgctgtcagg gagctcatgg agactaggtt tggccagaag gggaaggcaa tccggattga
     6181 gaagatagtg ttcacgggga aggaagggaa gagctctcag ggctgcccgg tcgccaagtg
     6241 ggtgatcaga agaagtggtc ctgaagagaa gcttatttgt ttggttcgtg agcgtgtaga
     6301 ccatcactgt tcgacggctg tgatagttgt ccttatcctg ctgtgggaag gtatccctcg
     6361 cctgatggct gaccgcctgt acaaagagct cactgagaac ttgaggtcct acagcggaca
     6421 tcccacagac cgaagatgta ccctcaacaa aaagcgtacc tgcacctgtc aaggcatcga
     6481 cccaaaaacc tgcggagcgt ccttctcctt tggctgttcg tggagcatgt atttcaacgg
     6541 ctgtaagttt gggaggagtg aaaaccccag aaaattcaga cttgctccaa actacccctt
     6601 acatgaaaaa cagcttgaaa agaatttaca agaattggct acagtattag ctccacttta
     6661 caagcagatg gctccagttg cttatcaaaa tcaggtggaa tatgaagaag ttgctggaga
     6721 ctgtcgactt ggaaatgaag aggggcgtcc tttctctggt gtcacctgtt gcatggattt
     6781 ttgtgcccat tctcacaagg acattcacaa catgcacaac ggaagcaccg tggtgtgtac
     6841 gttgattcga gcagatggcc gtgacacaaa ttgtcccgag gatgaacaac tccacgtcct
     6901 gccactatac cggcttgcag acactgatga atttggctcc gtggaaggga tgaaggccaa
     6961 aatcaaatct ggggccatcc aagtcaatgg gccaaccagg aagaggcgac tacgttttac
     7021 tgagcctgtt cctcgatgtg ggaagagggc caaaatgaag cagaaccaca ataaatcagg
     7081 ttcacacaac actaagagct tttcatcagc ctcatctact tctcacctag tgaaagacga
     7141 atctacagac ttctgtcccc tgcaggcttc ctccgcagaa acatctacct gtacgtacag
     7201 taaaacagcc tcaggtgggt ttgcagaaac aagtagtatt ctccactgca caatgccttc
     7261 tggagcacac agtggtgcta atgcagctgc tggggaatgt actggaacgg tgcagcctgc
     7321 cgaggtggct gctcatcctc accagtctct tcccacagcc gattctcccg ttcatgctga
     7381 gcctctcact agtccatctg agcagctaac ttctaaccag tcaaaccagc agctccctct
     7441 cctcagcaat tctcagaaac tggcttcctg tcaggtggaa gatgagcggc accctgaagc
     7501 ggatgagcct cagcaccccg aggacgataa cttgcctcaa cttgatgaat tctggtcaga
     7561 cagtgaggag atctacgccg atccttcctt tggtggcgtg gcgatagcac ccattcacgg
     7621 ctcggtgctc attgagtgcg ctcggaagga gcttcatgct accacctctt tgcgctcccc
     7681 caaacgaggg gtcccttttc gtgtgtccct tgtattctac cagcacaaaa gcctaaacaa
     7741 gcctaatcat ggttttgata tcaacaaaat taagtgtaaa tgcaaaaaag taacgaaaaa
     7801 aaagcccgca gaccgggagt gtcctgatgt atcccccgaa gccaatttat cacaccaaat
     7861 tccttctcga gttgcatcaa ccttaacccg agacaatgtt gttaccgtgt ccccatactc
     7921 tctcactcat gttgcgggac cctacaatcg ttgggtctaa gcggccgctc gataagcttg
     7981 atatcgaatt ccgccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc
     8041 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8101 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     8161 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     8221 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     8281 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     8341 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     8401 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     8461 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     8521 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     8581 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     8641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     8701 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     8761 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     8821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     8881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     8941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     9001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     9061 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     9121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     9181 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     9241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     9301 caagtaaagc ggccagctta tcgactcgcc gatagtggaa accgacgccc cagcactcgt
     9361 ccgagggcaa aggaatagtc gagaaattga tgatctatta aacaataaag atgtccacat
     9421 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
     9481 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
     9541 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
     9601 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatcctaa
     9661 gcttatcgat gatctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     9721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     9781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     9841 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     9901 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc aagcttggcg
     9961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    10021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
    10141 ctttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
    10201 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
    10261 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
    10321 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaagag
    10381 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
    10441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12361 ttttactttc accagcgttt ctgggtgagc aaaaacggga aggcaaaatg ccgcaaaaaa
    12421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
