LOCUS       sequence-198795-        3094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LasR protein in E. coli. The expression is controlled by a
            constitutive promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Guo S, Murray RM
  TITLE     Construction of Incoherent Feedforward Loop Circuits in a Cell-Free 
            System and in Cells.
  JOURNAL   ACS Synth Biol. 2019 Mar 15;8(3):606-610. doi: 
            10.1021/acssynbio.8b00493. Epub 2019 Mar 6.
  PUBMED    30790525
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00493"; journalName: "ACS Synth Biol"; date: 
            "2019-03-15- 15"; volume: "8"; issue: "3"; pages: "606-610"
COMMENT     Sequence Label: pSG86-J23151-LasR
FEATURES             Location/Qualifiers
     source          1..3094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1044..1703
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1110..1129)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1724..1818
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2225..2242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2359..2904)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2439..2458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcattgctgt tcttgatggc tagctcagtc ctaggtacaa tgcaagcggg cccaagttca
       61 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctgg
      121 ggagggtttc taatggcctt ggttgacggt tttcttgagc tggaacgctc aagtggaaaa
      181 ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc gaagatcctg
      241 ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt cggcaactac
      301 ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga cccgacggtc
      361 agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta ccagacgcga
      421 aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg gctgaccatg
      481 ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga agcggaaaac
      541 cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat gctcaaggac
      601 tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa accggtggtt
      661 ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac cagttgggag
      721 atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg aaatattcgg
      781 cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa tttgggtctt
      841 attactctct aataatgaac taacgcatga gaaagccccc ggaagatcac cttccggggg
      901 cttttttatt gcgcccttga gagtcgatgt ttgtcttctt gatcgggcac gtaagaggtt
      961 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1021 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1081 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1141 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1201 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1261 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1321 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1381 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1441 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1501 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1561 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1621 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1681 atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1741 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1801 ttttttattg gtgagaatcc aagcctccga tcaacgtctc attttcgcca aaagttggcc
     1861 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     1921 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     1981 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     2041 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcggtagcg
     2101 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     2161 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     2221 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     2281 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     2341 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     2401 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2461 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     2521 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2581 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     2641 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     2701 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     2761 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     2821 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     2881 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     2941 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     3001 ttgtaattct catgtttgac agcttatcat cgataagctt ccgatggcgc gccgagaggc
     3061 tttacacttt atgcttccgg ctgaagacaa ggca
//
