LOCUS       sequence-198861-        6349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains: complete oriC region of M. florum L1 (oriC4 fragment), 
            colE1 rep origin, recoded tetM and pac resistance genes, origin of 
            transfer of RP4 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight 
            TF, Rodrigue S
  TITLE     Development of oriC-Based Plasmids for Mesoplasma florum.
  JOURNAL   Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 
            10.1128/AEM.03374-16. Print 2017 Apr 1.
  PUBMED    28115382
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-03-17"; volume: "83(7). pii: 
            e03374-16. doi"; pages: " 10.1128/AEM.03374-16. Print 2017 Apr 1"
COMMENT     Sequence Label: pMflPT-o4
FEATURES             Location/Qualifiers
     source          1..6349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3343..3362
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3343..3361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3369..3386
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3549..3568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4063..4081
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            4139..4248
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagctccctt
     2341 tagtgagggt taatgttgtg actgggaaaa ccctggcgga attcggccag tctacatgta
     2401 ctctttttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta
     2461 agtgactatt taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa
     2521 tttgatagcg ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc
     2581 cagtttaaga atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt
     2641 gtatacctat ggttatgcat aaaaatcccg gtgataaaag tatttatcac tgggattttt
     2701 atgccctttt gggtttttga atggaggaaa tctagatgac tgaatacaaa ccaacagtta
     2761 gattggcaac aagagatgat gttccaagag cagtaagaac acttgctgca gcgtttgctg
     2821 attaccctgc aacaagacac acagttgacc cagatagaca tatagaaaga gttacagaat
     2881 tacaagaatt atttttaact agagtgggat tagacattgg taaagtttgg gttgctgatg
     2941 atggagctgc agttgctgtt tggacaacac cagaatcagt tgaggcaggt gctgtatttg
     3001 cagaaattgg ccctagaatg gctgaattat caggttcaag attagctgca caacaacaaa
     3061 tggaaggact tttagctcca catagaccta aagaaccagc ttggtttttg gcaactgtag
     3121 gcgtaagtcc agaccatcaa ggaaaaggat taggaagcgc tgtagtttta ccaggagtag
     3181 aagctgctga aagggcagga gttccagctt ttttagaaac aagtgcacca agaaatttac
     3241 cattctatga aagactagga tttactgtta ctgcagatgt tgaagttcca gaaggtccta
     3301 gaacttggtg tatgactaga aaaccaggtg cataagctgc agtaatacga ctcactatag
     3361 ggcatacgat ttaggtgaca ctatagtagg gataacaggg taatttagta ggtagggcta
     3421 tatggtcgtt atgcgatact gatgcataaa ggccgcgttg ctggcgtttt tccataggct
     3481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3901 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4021 caagcagcag attacgcgca gaaaaaaaag aactcaacgc gcaattaacc ctcactaaag
     4081 ggaacaaaag ctggagcgct gcaggaattc gatatcaagc ttatcgatac cgtcgacgcc
     4141 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     4201 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     4261 tggatacacc aaggaaagtc tacacgaacc atttggcaaa atcctgtata tcgtgcgaaa
     4321 aaggatggat atacagaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     4381 aagcgctggt acccagcgat gaggatgaac ctactcaggg acctgatgtg acgtgtcgtg
     4441 aacacgagcg tgtttaattt ttgatggttg tcaagttctt ttcattgtat tcaaaccccc
     4501 ttttaaagtt gataattctt gttaattata atataagtgc tggaaaaagt atagtttttt
     4561 attgttattt ttaagaaaat ttatccattg tgtttttata tgaacttttt caacattttt
     4621 tataaccttt tgtggaaaag tggaaaaaag atatataaat actaaactat tgctttttag
     4681 tgttattcat tatgtttatt tctttttaaa atattttttt atccacacat tgtggaaaac
     4741 ttttattatt atgtgtttta atttatttta tgaaggggaa ttaatatgga aacaaaagca
     4801 ctttgagaaa aattaattaa taagttaaag aaggaaaaat taatagacca agacattatt
     4861 gaagaatata ttgttacttc agaattaatt aaaatttcaa atacagagtt cgtaatcctt
     4921 gttagaagta atcttggtgt aactatttta aatgaattta aagaagtatt tgtgtacgaa
     4981 tttaaatcag ttttaaacag ttatgtgtct gttgattttt taactaaaga aatttttgag
     5041 aaaaatacaa aaaaagagaa taaaaaagaa cccataaata ctgttttatc tgaaaatgca
     5101 cttacttttg agaattttat agttgggtca agtaataaac aagctaattt agctgctaaa
     5161 aatgttgttg caaaccctgg tatgagtttt aatcctttat ttatttatgg agattcagga
     5221 ctgggtaaaa cacatttatt acaagctatt aaaaatcaag cagaattaaa tggaaaaaaa
     5281 gttttatatt taacatctga agaatttaca aaaagaattg ttaatgcttt aaacaaaggt
     5341 gatttaagtg aaatagaaga attaaaaact gagataaatt ctaatgaatt ctttatttta
     5401 gatgatgttc aatttttaag taaaaaagat aaaacaaacg agtttttctt taacattatt
     5461 aacaatttta ctgaaaatgg taaacaatta gttttttcta gtgataaaac tcctgaattg
     5521 ttaaatggtt ttgataaaag aatgataact agatttaatt caggtttatc aacacctatt
     5581 aatgctttag atattccaac tgccaaattg ataattgaag ctgaaattaa aaaacaaggt
     5641 ttaaaacaaa aaattaaaga agatgccgtt gtttacttag ctcaaaactt tagcgatgat
     5701 gttagaaaaa ttaaaggttt agttaataga ttacttttct ttggtattca aaacgattta
     5761 ggtcacataa ttgatttgga agatgttatt gacttattta aagatacacc ttcagctaat
     5821 ttaggattat taaatgttaa aaaaattaaa gaagttgttg ctaaaaaata tgatgttact
     5881 ataaaagcca tcgatggtaa agcaagaaca actgctataa aaaatgctag acatctttct
     5941 atgtattttg caaaaataat tttaaaccat acatcaactc aaattggtgc agaatttggt
     6001 ggaagagacc atagtacagt tttaagtgct atttcgcgta ttgaaaaatt aatatataaa
     6061 gaaaaagaat tcaaaaaaat agttgagtct ttaaaaaacg aaataatagg aaaatagatg
     6121 taaattcgca tctatttttt ttgtgtggaa aagaaattaa acttgttgaa taaaaagtca
     6181 tttaaacctt tatttaccgt tgttttatga gtgttttcca catatcaact gcattataaa
     6241 tattacttat ataaagaata atataatata taaaagcatg cgtatttctt ttgtttttat
     6301 tttataaata tgagataata tatattgtaa ataagaggtg ttatatgag
//
