LOCUS       sequence-198867-        2055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human codon optimized TtAgo; pCDNA3 vector backbone, mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ
  TITLE     Unexpected binding behaviors of bacterial Argonautes in human cells 
            cast doubts on their use as targetable gene regulators.
  JOURNAL   PLoS One. 2018 Mar 27;13(3):e0193818. doi: 
            10.1371/journal.pone.0193818. eCollection 2018.
  PUBMED    29584750
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-03-27"; volume: "13(3):e0193818. doi"; pages: " 
            10.1371/journal.pone.0193818. eCollection 2018"
COMMENT     Sequence Label: pCDNA-hTtAgo
FEATURES             Location/Qualifiers
     source          1..2055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaatcacc tgggaaagac cgaggtgttc ctgaacagat tcgccctgcg gcccctgaac
       61 cccgaggaac tgaggccttg gagactggaa gtggtgctgg accctccacc cggcagagaa
      121 gaggtgtacc ctctgctggc ccaggtggca agacgggctg gcggagtgac agtgcggatg
      181 ggagatggac tggccagctg gtccccacct gaagtgctgg tgctggaagg caccctggcc
      241 agaatgggcc agacctacgc ctaccggctg taccccaagg gcagaaggcc cctggaccct
      301 aaggatcccg gcgagagatc tgtgctgagc gccctggcta gacggctgct gcaggaaaga
      361 ctgcggcgcc tggaaggcgt gtgggtggaa ggactggccg tgtacagaag agagcacgcc
      421 agaggacctg gctggcgggt gctgggcgga gctgtgctgg atctgtgggt gtcagatagc
      481 ggcgccttcc tgctggaagt ggaccccgcc tacagaatcc tgtgcgagat gagcctggaa
      541 gcttggctgg ctcagggcca ccccctgcct aaaagagtgc ggaacgccta cgaccggcgg
      601 acctgggaac tgctgagact gggcgaagag gaccccaaag agctgcctct gcctggcgga
      661 ctgagcctgc tggattacca cgcctctaag ggccggctgc agggaagaga aggcggcaga
      721 gtggcttggg tggccgatcc caaggacccc agaaagccca tccctcacct gaccggactg
      781 ctggtgcctg tgctgaccct ggaagatctg cacgaggaag agggcagcct ggccctgtct
      841 ctgccctggg aggaaagacg cagacggacc agagagatcg ccagctggat cggcagacgg
      901 ctgggactgg gaacacctga agctgtgcgg gcccaggcct acagactgag catccctaag
      961 ctgatggggc ggagagccgt gtccaaacct gccgatgctc tgagagtggg cttctacaga
     1021 gcccaggaaa ccgctctggc cctgctgagg ctggatggcg ctcagggatg gcccgagttt
     1081 ctgagaaggg ctctgctgag agccttcggc gcctctggcg cttctctgag actgcacaca
     1141 ctgcacgccc accctagtca gggcctggcc tttagagagg ccctgcggaa ggccaaagaa
     1201 gagggggtgc aggctgtgct ggtgctgaca cctcctatgg cctgggaaga tcggaaccgg
     1261 ctgaaagccc tgctgctgag ggaaggcctg cccagccaga tcctgaacgt gcccctgcgg
     1321 gaagaggaac ggcacagatg ggagaatgcc ctgctgggcc tgctggccaa agctggactg
     1381 caggtggtgg ctctgtctgg cgcctatcca gctgagctgg ccgtgggctt tgatgccggc
     1441 ggaagagaga gcttcagatt cggcggagcc gcctgtgcag tgggcggaga tggcggacat
     1501 ctgctgtgga cactgcctga agcccaggcc ggcgaaagaa tcccccagga agtcgtgtgg
     1561 gatctgctgg aagagacact gtgggccttc agacggaagg ccggcagact gcctagcaga
     1621 gtgctgctgc tgcgcgacgg aagagtgccc caggatgaat tcgcactggc cctggaagcc
     1681 ctggcccggg aaggcattgc ctacgatctg gtgtccgtgc ggaagtccgg cggaggcaga
     1741 gtgtatcctg tgcagggcag actggctgac ggcctgtatg tgcctctgga agataagacc
     1801 tttctgctgc tgaccgtgca ccgggacttc agaggcaccc ccagacctct gaagctggtg
     1861 cacgaagccg gcgacacacc actggaagct ctggcacacc agattttcca cctgacccgc
     1921 ctgtaccccg ccagcggctt tgcctttcct agactgcctg cccctctgca cctggccgac
     1981 cggctcgtga aagaagtggg caggctgggc atccggcacc tgaaagaggt ggaccgcgag
     2041 aagctgttct tcgtg
//
