LOCUS       sequence-198869-        2661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human codon optimized NgAgo; pCDNA3 vector backbone, mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ
  TITLE     Unexpected binding behaviors of bacterial Argonautes in human cells 
            cast doubts on their use as targetable gene regulators.
  JOURNAL   PLoS One. 2018 Mar 27;13(3):e0193818. doi: 
            10.1371/journal.pone.0193818. eCollection 2018.
  PUBMED    29584750
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-03-27"; volume: "13(3):e0193818. doi"; pages: " 
            10.1371/journal.pone.0193818. eCollection 2018"
COMMENT     Sequence Label: pCDNA-hNgAgo
FEATURES             Location/Qualifiers
     source          1..2661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..2661
                     /codon_start=1
                     /product="Argonaute protein from Natronobacterium gregoryi 
                     (Gao et al., 2016)"
                     /label=NgAgo
                     /note="reportedly a DNA-guided endonuclease that does not 
                     require a PAM site, although this interpretation has been 
                     challenged (Cyranoski, 2016)"
                     /translation="MTVIDLDSTTTADELTSGHTYDISVTLTGVYDNTDEQHPRMSLAF
                     EQDNGERRYITLWKNTTPKDVFTYDYATGSTYIFTNIDYEVKDGYENLTATYQTTVENA
                     TAQEVGTTDEDETFAGGEPLDHHLDDALNETPDDAETESDSGHVMTSFASRDQLPEWTL
                     HTYTLTATDGAKTDTEYARRTLAYTVRQELYTDHDAAPVATDGLMLLTPEPLGETPLDL
                     DCGVRVEADETRTLDYTTAKDRLLARELVEEGLKRSLWDDYLVRGIDEVLSKEPVLTCD
                     EFDLHERYDLSVEVGHSGRAYLHINFRHRFVPKLTLADIDDDNIYPGLRVKTTYRPRRG
                     HIVWGLRDECATDSLNTLGNQSVVAYHRNNQTPINTDLLDAIEAADRRVVETRRQGHGD
                     DAVSFPQELLAVEPNTHQIKQFASDGFHQQARSKTRLSASRCSEKAQAFAERLDPVRLN
                     GSTVEFSSEFFTGNNEQQLRLLYENGESVLTFRDGARGAHPDETFSKGIVNPPESFEVA
                     VVLPEQQADTCKAQWDTMADLLNQAGAPPTRSETVQYDAFSSPESISLNVAGAIDPSEV
                     DAAFVVLPPDQEGFADLASPTETYDELKKALANMGIYSQMAYFDRFRDAKIFYTRNVAL
                     GLLAAAGGVAFTTEHAMPGDADMFIGIDVSRSYPEDGASGQINIAATATAVYKDGTILG
                     HSSTRPQLGEKLQSTDVRDIMKNAILGYQQVTGESPTHIVIHRDGFMNEDLDPATEFLN
                     EQGVEYDIVEIRKQPQTRLLAVSDVQYDTPVKSIAAINQNEPRATVATFGAPEYLATRD
                     GGGLPRPIQIERVAGETDIETLTRQVYLLSQSHIQVHNSTARLPITTAYADQASTHATK
                     GYLVQTGAFESNVGFL"
ORIGIN
        1 atgacagtga tagatctgga ttcaacaaca accgcagacg agcttacgtc tggacatact
       61 tatgatatca gcgtcactct taccggggtc tacgataaca ctgacgagca gcacccccgc
      121 atgtcccttg ctttcgaaca agacaatggc gagcgacgct acataaccct ctggaagaac
      181 acaactccca aggacgtctt cacttacgat tatgccacag gcagtacgta tattttcact
      241 aatattgact acgaggtcaa ggacggttat gaaaacctta ccgccaccta ccaaacaacg
      301 gttgaaaatg ctacagctca agaagtcggc accacggatg aagacgaaac atttgccggg
      361 ggcgaaccat tggatcacca cctggatgat gcactgaacg agacccccga tgatgcggaa
      421 acggaaagtg attcaggcca tgtaatgaca tcattcgcgt cacgcgatca gctgccggag
      481 tggacattgc acacatacac tctcacggcc acggatgggg ctaaaacaga tacggaatac
      541 gcgagaagaa ctctggctta cactgttcgg caagaacttt ataccgacca cgacgcagca
      601 ccagtggcca ccgacggctt gatgcttctc actcctgagc ctctgggaga gacccctctt
      661 gatcttgatt gtggcgtgcg cgttgaggct gacgagacaa ggacgttgga ttacacaacc
      721 gcaaaagata gacttctcgc tcgggaactt gtggaggagg ggttgaaacg gtctctttgg
      781 gacgattacc tcgtgagggg catagatgaa gtcctgtcaa aggagcccgt gttgacttgt
      841 gatgagtttg acctgcacga gcgctatgac ctgtctgtcg aggttggtca ttctggccgg
      901 gcttatcttc acattaactt ccgccaccgc ttcgtaccga agctcacgct tgcagacata
      961 gatgacgaca acatttatcc ggggttgcgg gtgaaaacaa cctacagacc tcgccgaggc
     1021 catatagtct ggggtctccg cgacgaatgt gcgaccgact cccttaatac tttgggtaat
     1081 cagtcagtag tagcttatca ccgcaataac cagacaccaa tcaataccga ccttctcgat
     1141 gctattgaag ccgcagaccg cagagtcgtc gaaacgcgca ggcaaggaca tggggatgac
     1201 gccgtatcat tcccccagga gcttctcgca gtagaaccta atacacatca aataaagcag
     1261 ttcgcctccg acgggtttca tcagcaggct aggtctaaga cccgactcag tgcatcaaga
     1321 tgttcagaaa aagcacaagc atttgcggag cggcttgacc ctgtgcgatt gaacgggtct
     1381 acggtggaat tctcttctga gttcttcacg gggaacaacg agcaacagct ccggttgttg
     1441 tacgaaaacg gcgaatccgt gttgaccttt cgggatggtg ccaggggggc ccatcccgac
     1501 gaaacattta gtaagggaat agtcaatcca ccagagtcat ttgaggtagc agtcgtgctg
     1561 cctgaacagc aggctgacac atgtaaagcc cagtgggaca ctatggcaga cctcttgaat
     1621 caggcgggcg ctcctcctac tcgatcagag acggttcaat atgatgcgtt ctcttctcct
     1681 gaaagtattt ctctcaatgt agccggtgcg atagacccgt cagaagtcga tgcagcgttc
     1741 gtcgttctgc cccctgatca ggagggtttc gctgatcttg cttctccaac cgaaacctat
     1801 gacgaactga aaaaagcact tgcgaacatg ggcatttatt ctcaaatggc atacttcgat
     1861 cgctttcggg acgctaagat tttctacacc aggaacgttg ccttgggttt gctggcagca
     1921 gcgggcggcg tcgcttttac gacagaacac gcgatgcctg gagatgcgga catgtttatt
     1981 ggaattgatg tgagccgaag ctatccggaa gatggggcgt caggtcagat taatatagcg
     2041 gctaccgcga cggccgtcta taaggacggt acgatattgg gtcactcttc cacgaggcct
     2101 caactcggag aaaagctcca atcaactgac gtcagagata taatgaagaa tgcgatattg
     2161 ggctatcagc aagtgacggg tgaatcccct acccatattg ttatccatcg cgacggattt
     2221 atgaacgaag atttggatcc cgcgactgag tttctcaacg aacagggtgt ggagtatgac
     2281 attgtggaga ttaggaaaca accccaaaca cggctcttgg ccgtgtcaga cgtccagtac
     2341 gatacgcctg tgaagtcaat tgcagccatc aatcagaacg aaccccgggc gaccgttgcg
     2401 acctttggag ctccggagta tcttgcgact cgcgatggcg gagggttgcc gagaccgata
     2461 cagatagaga gggtcgccgg cgaaacggat atagaaacgc tcacacgcca agtatacctt
     2521 ctttctcaat ctcatatcca ggtgcacaac tcaacggctc gcttgcccat aacgacggca
     2581 tacgctgacc aggcgtcaac gcatgcgacg aagggttatc tggtacaaac gggggcgttc
     2641 gagagtaacg tcggtttttt g
//
