LOCUS       sequence-198997-        9483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ariumi Y, Turelli P, Masutani M, Trono D
  TITLE     DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable 
            for human immunodeficiency virus type 1 integration.
  JOURNAL   J Virol. 2005 Mar . 79(5):2973-8.
  PUBMED    15709017
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2005 Mar . 79(5):2973-8."
COMMENT     Sequence Label: pLV.ATMi
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..78
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        234..563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      414..549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             573..1172
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(573..592)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1069..1089
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1243..1831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1296..1316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1714..1725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1924..2157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2182..2200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2183..2200)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2490..2509)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2723..2742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2842..2864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2902..2920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2988..3092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3093..3161,3162..3953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3311..3330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4124..4712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4613..4632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4866..4883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4958..5287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5138..5273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6421..6486
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6616..6636
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7061..7195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7098..7117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7152..7171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7363..7996
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8043..8168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8661..8894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9079..9123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(9407..9483,1..138)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
ORIGIN
        1 ccctggcagg aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc
       61 gctatgtgtt ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata
      121 agttctgtat gagaccacag atccccggat ttgcgtatta ctcagttcaa gagactgagt
      181 aatacgcaaa tcctttttgg aaaagcttat cgataccgtc gaccctgtgg aatgtgtgtc
      241 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      301 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      361 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
      541 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
      601 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
      661 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
      721 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
      781 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
      841 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
      901 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
      961 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     1021 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     1081 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     1141 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     1201 ccgaaaggag cgcacgaccc catgcatcga attaattccg ataatcaacc tctggattac
     1261 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1321 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1381 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1441 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     1501 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     1561 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     1621 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     1681 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     1741 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     1801 agtcggatct ccctttgggc cgcctccccg catcgggaat taattcgagc tcggtacctt
     1861 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     1921 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     1981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     2161 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     2221 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     2281 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     2341 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     2401 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     2461 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     2521 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     2581 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     2641 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     2701 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2761 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2821 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2881 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2941 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3061 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4921 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     4981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5221 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5281 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     5341 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     5401 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     5461 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     5521 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     5581 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     5641 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     5701 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     5761 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     5821 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     5881 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     5941 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     6001 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     6061 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     6121 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     6181 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     6241 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     6301 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     6361 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     6421 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     6481 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     6541 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     6601 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     6661 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     6721 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     6781 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     6841 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     6901 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     6961 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7021 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     7201 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     7261 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     7321 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     7381 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     7441 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     7501 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     7561 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     7621 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     7681 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     7741 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     7801 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7861 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7921 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7981 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8041 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8101 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8161 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8221 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8281 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8341 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8401 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8461 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8521 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8581 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8641 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8701 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8761 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8821 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8881 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8941 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9001 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9061 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9121 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9181 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9241 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9301 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9361 gtgaacggat ctcgacggta tcggatcccc cgggctgcag gaattcgaac gctgacgtca
     9421 tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt
     9481 gcg
//
