LOCUS       sequence-199095-        5116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E.coli/H.Pylori shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Heuermann D, Haas R
  TITLE     A stable shuttle vector system for efficient genetic complementation
            of Helicobacter pylori strains by transformation and conjugation.
  JOURNAL   Mol Gen Genet. 1998 Mar;257(5):519-28.
  PUBMED    9563837
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Gen 
            Genet."; date: "1998-03"; volume: "257(5)"; pages: "519-28"
COMMENT     Sequence Label: pHel2
FEATURES             Location/Qualifiers
     source          1..5116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2751..3410
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2817..2836)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3539..3587
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      complement(3860..4448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3940..3959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4674..4783
                     /note="incP origin of transfer"
ORIGIN
        1 aactatgttg tttttacatg tttttacaac gcgcgcgcgt gtgagggatt gggggttgca
       61 accccctaaa taacgaagct gtagggtttc tcatttttgg gttaaaatga ataaaacaga
      121 acttcttacg agtgataaca gaacttctta cgagtgataa cagaacttct tacgagtgat
      181 aacagaactt cttacgagtg ataacagaac ttctttattg tgaagttatg attattaaca
      241 atttttagct aaaataacaa cctaaggcgg tgtaacatgg ttgctaatcc tagtgttaac
      301 aaatttggag caattagctt taaaagctag tgggttggga gtttgtagcg ggtagcactc
      361 cgttaggagg cacaccatga aagcattttt gatagtagtg attttagtgg taatcttgac
      421 acagccacta tattaaaacc ttagcgtttt aataaccctt ataagtccgc caagacttct
      481 taagggtttc actcctgtta ttatatcgtc ttttgaaaaa taagcattaa aaggcgctta
      541 aatgcccatg aatacgaatt ttgaacagct tagaaaacaa gaattggaat tacgaaaatt
      601 attagaagaa ttagaaacgc tcccacaaac cccacaaatt aaactgcaaa aacaaaaaat
      661 acaaacttac atagacaaga taacaccaag tattttgagc ggttttgatc aaaaattcaa
      721 agaaattata gaaaatctat caaatgaatt tgaaaaagaa aaatccacac cactcaaaga
      781 gccacaaacc acccccacac catgcaaaga tttagtggtt agcaccccta aagataacac
      841 ctataccacc taccacaata acgctaataa ggtcaatcta gggaaattga gcgaaaggga
      901 agccaatctt ttattcgcta tttttcaaaa actcaaagat caagggaata ccctcattcg
      961 ttttgaaccg caagatttga aacgcatgat catagtcaaa tccaacctaa ccaacagaca
     1021 attattgcaa atcctcaaaa acttacttga caacataggc ggtgctaatt tttggatcat
     1081 tagagagcat gttgaaaatg gcgaaatcta tgaagatcac actagctaca tgcttttcaa
     1141 acaatttgac attcgtatcc ataagccaac ccaaactata gaatacttag aagtccaact
     1201 caacgatagc taccactacc tgctcaacaa tctaggcatg agcggtcaat acacttcctt
     1261 taaactttta gaattccaaa gggtgagggg caaatacgcc aagacgctct atcgcttact
     1321 caagcaatac aaaagcacag ggattttgag cgtggaatgg gatcaattca gggagctttt
     1381 agacattcca aaagattaca aaatggaaaa catcgatcaa aaagtcttaa ctccaagcct
     1441 caaggaactc agaaaaatct atccctttga acacttgagc tacaaaaaag aacgcaaaag
     1501 ccacgacaaa cgcaaggtaa cgcatattga tttttatttt gagcaattgc cacagggcga
     1561 aaccaagaaa caaaaacaag ccgacaagca acgcgctcaa agggacatca agctcatagc
     1621 gtgggatatt cacaaccaaa tcgctaaaag aaacgctaaa gccactatag aagctaggtt
     1681 tcttgagcta aaaaccttga ttggttatca gttcaggcac aacaatggga ctattttaca
     1741 gattgacaac gccacttttg aaaagaatca aatgtttttg catgtttcaa ccagcaaaaa
     1801 ttctcaaaaa ttccttgtgt ccaacaaaac attcgcttta gaacttctgt ttgtcaatgg
     1861 atacagccta aaaaaagaca tgttggaaat tgatccccca agtatccacc ccatcaccaa
     1921 cgaacctatc aaggaatttg cagaatacat cggaaaaacg atccacatca ccaatttcaa
     1981 tgtggatcaa tgccctgagg ggatcaataa ctacctgaaa atcactagga ttgtgaaact
     2041 gaatgacaat cggatctgtg tttcagtcca agatgtggat aagcctgaga aacttctaaa
     2101 acctttcatt gctaaagatg aaaaacattt gaaaaattgg tttaagaaac actatcggtg
     2161 aaaaataagt ggtgggatta acaatcgtcc cccacacctt gcaatgtagt tagttttgca
     2221 ttattttttc tataatctct atcaattctg agagaacata gattaattca atgaaatttt
     2281 tcattatcta tttcctttct gtaaattttg ttaaatattt attaaaaaaa aatttaaaga
     2341 tcactcttta agtggaacat acaaaggctg tagagagtgc cacattaagc ctgatgtttt
     2401 gcttgtgtat agagtgaaag gcaatatttt aactttagtt aggcttggca gtcatagtga
     2461 attgttttag acaaatttct ttctaaaatc gcccagaatg aatttttaat tctaatttaa
     2521 tataattcat cggttttcac tcaaaacgat gaaatagggc tatttttagc tcattattgg
     2581 ggtattttgt ttttagttga aagctaactt caaaattcaa aaaaacttca tttccctttc
     2641 tagagatccg gtttttgtta atccgccata ttgtgttgaa acaccgcccg gaacccgata
     2701 taatccgccc ttcaacagat ccgagatttt caggagctaa ggaagctaaa atggagaaaa
     2761 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     2821 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     2881 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     2941 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     3001 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     3061 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     3121 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     3181 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     3241 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     3301 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     3361 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     3421 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
     3481 tgaatggcag aaattcggat cttccatacc taccagttct gcgcctgcag gtcgataaac
     3541 cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa cgtggatctg
     3601 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagctagctt
     3661 tcgcgagctc gagatctaga tatcgatgaa ttcaaaaaaa aaggctccaa aaggagcctt
     3721 taatgatcta attcgtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4201 caccggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4441 gatctcaaga agatcctttg atctctgcag ttcagtaatt tcctgcattt gcctgtttcc
     4501 agtcggtaga tattccacaa aacagcaggg aagcagcgct tttccgctgc ataaccctgc
     4561 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     4621 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     4681 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     4741 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca
     4801 gatgagggca agcggatggc tgatgaaacc aagctgcaga agcttgtccg ttagcaaata
     4861 taatgtgttt agcacctaaa agcgcatcta tcttgtgtta gccatctaac ctaaacaaaa
     4921 ccgcctagcg agcgttagcg agcatggaca aaagcgcatc gcagtttgaa atcgtaggcg
     4981 ttagccggag atggttttgc gttagcaaaa tcaaacaaga tagcgcaaac cgaggcgtca
     5041 gccaaaaaaa ccctaaaact aaaatcccca aaatatgtag cgcgtcatgc gcgttgttta
     5101 tattacatgt tttaat
//
