LOCUS       sequence-199114-       10816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. pombe gRNA entry vector with Cas9 - KanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     Construction of Designer Selectable Marker Deletions with 
            CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized 
            Design of Common Entry Vectors.
  JOURNAL   G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363.
  PUBMED    29321167
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 
            10.1534/g3.117.300363."
COMMENT     Sequence Label: pYZ294
FEATURES             Location/Qualifiers
     source          1..10816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..742
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             763..4866
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4879..4899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4915..5162
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4931..4949)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     5209..5227
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5265..5287)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5387..5406
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      5777..6558
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     gene            6703..8058
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             7047..7856
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7114..7133)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7861..8058
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     8059..8075
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(8125..8176)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(8269..8625)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(8662..8678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8662..8678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8675..8697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8686..8702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8710..8716,8717..8734,8735..8740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8755..8776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8893..8910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9064..9652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9144..9163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9823..10614,10615..10683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10446..10465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10684..10788)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccctacaac aactaagaaa atggctatca tgcggaagct ggtgagaaga acagcatcgg
       61 gacaagggaa ggaagaacaa agacaaagaa aacaaaagaa agcaattgaa aacaaaacaa
      121 aacaattttc attccttctc ttatcattcc ttttcttttc ttttctctca ttcaacgcac
      181 tccatcgtat ccgtattcct cttatttttt ctctttctct atatccattt ctttctctct
      241 aggtgtgtcc tctctctctc ttcaatttct ctactccgca ttccaacgca tccttccccc
      301 aacctcccat ttcctcctta cggcccgata gcgatcgtct ttccctcgct atcactcgct
      361 accggcccct cctctgcacc gtaacctcct acgtatttac catatcataa agttttttcc
      421 gacgcttatc gctgaccccc tgtcgccctc ctattggctt ccggattatc ttcttgtcca
      481 taaggtgatc catgcttcct gaagattccc gaaatgtgtc cactttggcg gggaatcatt
      541 ccatccactt ctttctctct cgctttcctc attcggcgct ccccttccgc gtctcattgg
      601 tcttccgctc cgtttttgct ttgccgatgt tacttgggga gaggtgcgat aatcctttcg
      661 caaaaactcg gtttgacgcc tcccatggta taaatagtgg gtggtggaca ggtgccttcg
      721 cttttcttta agcaagagaa ttgctgcagg tcgactctag agatggacaa gaagtactcc
      781 attgggctcg atatcggcac aaacagcgtc ggttgggccg tcattacgga cgagtacaag
      841 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
      901 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
      961 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
     1021 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
     1081 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
     1141 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
     1201 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
     1261 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
     1321 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
     1381 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
     1441 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
     1501 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
     1561 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
     1621 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     1681 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
     1741 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     1801 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     1861 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     1921 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     1981 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     2041 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     2101 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     2161 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
     2221 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     2281 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     2341 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
     2401 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     2461 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     2521 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     2581 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     2641 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     2701 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     2761 atcaatggga tccgagataa gcagtctgga aagacaatcc tggattttct taagtccgat
     2821 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag
     2881 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
     2941 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     3001 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     3061 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     3121 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     3181 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     3241 gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc ccagtctttt
     3301 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
     3361 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     3421 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     3481 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     3541 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     3601 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
     3661 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     3721 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     3781 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     3841 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     3901 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     3961 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     4021 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     4081 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     4141 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     4201 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     4261 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     4321 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     4381 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
     4441 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     4501 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     4561 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     4621 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     4681 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     4741 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     4801 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     4861 ggagacagca gggctgaccc caagaagaag aggaaggtgt gatctcttct cgagtcatgt
     4921 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     4981 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     5041 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     5101 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     5161 gcgatccccg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5401 attgtactga gagtgcacca taccatcgat ggccaacctt ccaattcatt aaatcgagtc
     5461 ctccatcaga tgatttacaa ggttttgcat agaatcccta agtaagagaa gttgttagtg
     5521 atttcttcaa gtttactaaa atttacatac aaatggatga tctcttctcc attgtttcct
     5581 ttcttcttgc aaacgcgttt tacaaagaga tgacatgcta ttttctgatt atttttttct
     5641 atagttttct attttgtacg tccttgggag tccgaaatgt aaaatcgggg tatggttagt
     5701 agcggcagcg ttcattatgg tatataaaag ataaaaagta ggggcaagcg agacagaatg
     5761 ggatacaagg gcatcgtcta taattatagc taaaaattgt attttaattt gtattttttg
     5821 taattttatt tctcattgtt ttacttaaaa tgaatgcgaa ttagagaaaa cttcaacgaa
     5881 atgtcaaaat aagctcagca aaatatacaa ttttagggaa agcgatcagc aacttattct
     5941 ccgttgtaga ttctttttct tcataaattc caacaatgta actgccaatt gatttttctc
     6001 tagatgctaa taaattgaaa ctaatgaata taataacaca ggttttgata aattgtaaac
     6061 tcttactcaa aaaaagaatg ttaaaactaa tttaaccttt ttataaaggc tcatggcgtt
     6121 aaagaggata gtaaaaattc tacaattttt taatttaatg tcacaattgt cttgcgaata
     6181 taattctatt aattttcgct aactgctgtt ttagtactat tactttcaat atgccttata
     6241 tgcaacttta attcatgaag aatgcaatat ttttgcaatc tttcttgtac tgagcgcttt
     6301 ttatcattta gtactcatta tttaattttt cagtaaaggg caataaataa atttgtagaa
     6361 gatgcaatgt aatctcctct atccttttgc tcatatgttt atgagtatac ctagtctaga
     6421 aaggcttgta tttaaaatat tattcaataa aaattcacaa tttttacgac atgtgctacc
     6481 attcacttaa cttcctgatt ataaaattgg ttcgtttata ctaattactt aagtaccttt
     6541 aactaaacaa aatgcctata tatatattaa tttacaatga gtgtcagata agtcactatg
     6601 tccgagtggt taaggagtta gactcgccat cgatgagctt agctacaaat cccactggct
     6661 atatgtaagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
     6721 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
     6781 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
     6841 gcgcgaagca aaaattacgg ctcctcgctc cagacctgcg agcagggaaa cgctcccctc
     6901 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
     6961 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
     7021 cacatcacat ccgaacataa acaaaaatgg gtaaggaaaa gactcacgtt tcgaggccgc
     7081 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     7141 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     7201 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     7261 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     7321 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc
     7381 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     7441 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctggct caggcgcaat
     7501 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     7561 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
     7621 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     7681 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     7741 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     7801 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaaagta
     7861 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     7921 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     7981 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     8041 atgctggtcg ctatactggt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     8101 actctagagg atccccacgg tacccctgtt tcgtcctcac ggactcatca gaccggaaag
     8161 cacatccggt gacagggcac caccgactcg gtgccactca cttttttcaa gttgataacg
     8221 gactagcctt attttaactt gctatttcta gctctaaaac gcggccgctt cttcggtaca
     8281 ggttatgttt tttggcaaca gcaactattc aaatcaactg aaagtttttt agaattcaac
     8341 aacttccaat actcaagtgt ccaagctagc aaactgttta ataatcgtga cccaacattc
     8401 ctttcatgcg caccctcagc gtcacataaa tcaacacttt acaacaccta ttcaaagctt
     8461 ctatatgcct tcgtcattta aatgtctaac atttcaaaaa agtgtttgct aatggtaaat
     8521 tcttgagttt tcttggaagc acatatataa cgagtgtgac tgttctacgc tccttgctga
     8581 ctagtcatca gtctacgcat gccaactacc accaacataa gcaaaaggta cccggggtac
     8641 cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8701 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8761 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8821 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8881 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9061 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9121 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9181 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9241 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9301 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9361 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9421 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9481 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9541 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9601 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9661 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9721 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9781 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10801 aaaagtgcca cctgac
//
