LOCUS       sequence-19913-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K
  TITLE     Vectors for co-expression of an unrestricted number of proteins.
  JOURNAL   Nucleic Acids Res. 2007;35(6):e43. doi: 10.1093/nar/gkm067. Epub 
            2007 Feb 20.
  PUBMED    17311810
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35(6):e43. doi"; pages: " 
            10.1093/nar/gkm067. Epub 2007 Feb 20"
COMMENT     Sequence Label: pQLinkHD
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(81..95,96..101,102..118,119..125)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    101..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    133..149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             168..179
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             192..212
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    225..349
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gcttaattaa
       61 caacaccatt tgtcgagaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta
      121 taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa gaggagaaat
      181 taactatgaa acatcaccat caccatcacc atggtgctgc aggtacaagt ttgtacaaaa
      241 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      301 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatga atcaactact
      361 tagatggtat tagtgacctg tagtcgaccg acagccttcc aaatgttctt cgggtgatgc
      421 tgccaactta gtcgaccgac agccttccaa atgttcttct caaacggaat cgtcgtatcc
      481 agcctactcg ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag
      541 gtgcgagcct cttttttgtg tgacaaaata aaaacatcta cctattcata tacgctagtg
      601 tcatagtcct gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt
      661 acaagtcgtt cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt
      721 tctgtaattt ctactgtatc gacctgcaga ctggctgtgt ataagggagc ctgacattta
      781 tattcc
//
