LOCUS       sequence-199362-        7246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Excitatory presynaptic marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Kwon SK, Sando R 3rd, Lewis TL, Hirabayashi Y, Maximov A, Polleux F
  TITLE     LKB1 Regulates Mitochondria-Dependent Presynaptic Calcium Clearance 
            and Neurotransmitter Release Properties at Excitatory Synapses along
            Cortical Axons.
  JOURNAL   PLoS Biol. 2016 Jul 18;14(7):e1002516. doi: 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul.
  PUBMED    27429220
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2016-07-18"; volume: "14(7):e1002516. doi"; pages: " 
            10.1371/journal.pbio.1002516. eCollection 2016 Jul"
COMMENT     Sequence Label: pCAG vGlut1 mCherry
FEATURES             Location/Qualifiers
     source          1..7246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(800..1591,1592..1660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1423..1442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1661..1765)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2177..2454
                     /label=chicken beta-actin promoter
     intron          2455..3467
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3391..3413
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3475..3494
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3528..3537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4819..4830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5229..5939
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5374..5392)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5642..5661
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6030..6049)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6095..6150
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6149..6168)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6541..6557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6541..6557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6554..6576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6565..6581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6589..6595,6596..6613,6614..6619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6634..6655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6714..6909
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6760..6895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6915..7049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6952..6971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7006..7025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7117..7134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgaggcc accatggagt
     3541 tccggcagga ggagtttcgg aagctggcgg ggcgcgccct ggggaggctg caccggttac
     3601 tggagaagcg gcaggaaggc gcggagacat tggagctgag cgccgacggg cgcccagtga
     3661 ccacacacac gcgggacccg ccggtggtgg actgcacttg ctttggcctc cctcgccgct
     3721 acatcatcgc gatcatgagc ggtctgggtt tctgcatcag ctttggcatc cgctgcaacc
     3781 tgggcgtggc catcgtatcc atggtcaaca acagtacaac ccaccgtggg ggccacgtgg
     3841 tggtgcagaa agcccagttc aactgggatc cagagactgt cggcctcata catggctcct
     3901 ttttctgggg gtacattgtc actcagattc ctggaggatt tatctgccaa aaattcgcag
     3961 ccaacagggt ctttggcttt gccattgtgg ctacctccac cctaaatatg ttgatccctt
     4021 cagcagcccg tgttcactat ggctgtgtca tcttcgtgag gatccttcag ggattggtgg
     4081 agggggtcac ataccctgct tgccatggca tctggagcaa atgggcccct cccttagaac
     4141 ggagtcggct ggcgacgaca gccttttgcg gttcctatgc cggggcagtg gttgccatgc
     4201 ctctggctgg ggtcctggta cagtattcag gatggagttc tgtcttctat gtctatggca
     4261 gcttcgggat cttttggtac ctgttctggt tgcttgtctc ctacgagtca cctgcactac
     4321 accccagcat ctccgaggag gagcgcaaat acattgagga tgccatcgga gaaagcgcca
     4381 agctcatgaa ccctgttacg aagtttaaca caccctggag gcgcttcttt acctccatgc
     4441 cggtctatgc catcattgtc gccaactttt gccgcagctg gactttctac ctgctcctca
     4501 tctcccagcc cgcctacttt gaagaagtgt tcggctttga gatcagcaag gtgggactgg
     4561 tgtcggcact gcctcacctt gtcatgacta tcatcgtacc catcggaggc cagatcgccg
     4621 acttcctgcg cagtcgtcat ataatgtcca cgaccaatgt gcgaaagctg atgaactgcg
     4681 ggggtttcgg gatggaagct acgctgctgc tggtggtcgg atactcacac tccaagggcg
     4741 tggccatctc cttcctggtc ctggctgtgg gcttcagtgg ctttgctatc tctgggttta
     4801 acgtgaacca cttggacatc gcccctcgat atgccagcat cttgatgggc atttccaatg
     4861 gcgtgggcac actgtctggg atggtgtgcc ccatcatcgt gggtgcaatg accaagcaca
     4921 agacgcggga ggagtggcag tacgtgttcc tcatagcctc cctggtgcac tatggaggtg
     4981 tcatcttcta tggggtcttt gcttcgggag agaaacagcc gtgggcagag ccggaggaga
     5041 tgagcgagga gaagtgtggc tttgttggcc acgaccagct ggctggcagt gacgaaagtg
     5101 aaatggaaga cgaggttgag cccccggggg caccccccgc acctccgcct tcctacgggg
     5161 ccacacacag cacagttcag cctccaaggc ccccaccccc tgtccgggac tacgaattcg
     5221 gtggttctat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     5281 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     5341 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     5401 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     5461 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     5521 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     5581 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     5641 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     5701 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5761 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     5821 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     5881 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagg
     5941 cggccgcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     6001 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     6061 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     6121 atagtgtgtt ggaatttttt gtgtctatca ctcggaagga catatgggag ggcaaatcat
     6181 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     6241 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     6301 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     6361 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     6421 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     6481 gacctgcacc gtcgaccagc tggtcgacgg tgcaccgtcg accagcttgg cgtaatcatg
     6541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6661 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     6721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6901 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6961 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7021 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     7081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccag
//
