LOCUS       sequence-199401-        9710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 2 - synthetic promoter E2F1 drives sponge 7 ,""optimized 
            sponge"" expression. (see PMID: 29056342 for detailed information).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN397 (FuGW-S(E2F1)p-Sponge7)
FEATURES             Location/Qualifiers
     source          1..9710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        393..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             609..789
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    836..961
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1454..1687
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1872..1916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2807..2816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2858..2879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3119..3138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3731..3982
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3919..3940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4176..4764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4229..4249)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4647..4658)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4767..4783)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4768..4784)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5289..5469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5495..5512)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5501..5725
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5771..6199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5858..5877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6068..6089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6208..6228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6213..6542
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6393..6528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6455..6474
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6590..6637
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6656..7030
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7160..7281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7197..7216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7251..7270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7330..7346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7330..7346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7343..7365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7354..7370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7378..7384,7385..7402,7403..7408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7423..7444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7561..7578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7732..8320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7812..7831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8491..9282,9283..9351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9114..9133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9352..9456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9531..9550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      601 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      781 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      841 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      901 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      961 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1021 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1081 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1141 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1201 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1261 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1321 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1381 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1441 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1501 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1561 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1621 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1681 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1741 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1801 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1861 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1921 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1981 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2041 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2101 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2161 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2341 tacagggaca gcagagatcc agtttggtta atggtaccct gcactggaca cccttggaag
     2401 caaatcccct gcaggtacgc gcgaaaactg agcgcgccaa aatcgctgag cgcgaaacgg
     2461 acggggcggg aagacttacg cgcgaaaact ggtcgcgcca aagaggctga gcgcgaaacg
     2521 tccggggcgg gaagcgaatt ctgccctact gacactgcct gccgtacgcg cgaaaactga
     2581 gcgcgccaaa atcgctgagc gcgaaacgga cggggcggga agacttacgc gcgaaaactg
     2641 gtcgcgccaa agaggctgag cgcgaaacgt ccggggcggg aagcgaatat ccgcggcgcg
     2701 ccagacgcta gcggggggct ataaaagggg gtgggggcgt tcgtcctcac tctagatctg
     2761 cgatctaagt aagcttggca ttccggtact gttggtaaac cagctcgcca ccatggtgag
     2821 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2881 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2941 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3001 caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     3061 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3121 cgacggcaac tacaagaccc gcgccgagct tcctgcctga cacagctcac ttcaagaagt
     3181 gcacatgtca gaacgtggaa ttaagtgcat ggacgagctg tacaagtaaa ggatccgctt
     3241 gaaggaataa ttaaagctag ccgcttgaag gaataattaa agatagccgc ttgaaggaat
     3301 aattaaagct agccgcttga aggaataatt aaagctagcc gcttgaagga ataattaaag
     3361 cttgtaagcg ggactctggg gttgaattcg gatccgcttg aaggaataat taaagctagc
     3421 cgcttgaagg aataattaaa gatagccgct tgaaggaata attaaagcta gccgcttgaa
     3481 ggaataatta aagctagccg cttgaaggaa taattaaagc ttgtaagcgg gactctgggg
     3541 ttaaagcaaa ttaaaaacag gatgaaaaag actgattggc tcttcctcaa tgcttgtgtg
     3601 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3661 ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatcagcg acaacgtcta
     3721 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     3781 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3841 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     3901 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3961 cggcatggac gagctgtaca agtaaggatc cgcttgaagg aataattaaa gctagccgct
     4021 tgaaggaata attaaagata gccgcttgaa ggaataatta aagctagccg cttgaaggaa
     4081 taattaaagc tagccgcttg aaggaataat taaagcttgt aagcgggact ctagggttcg
     4141 aaatgaccga ccaagccgat atcaagctta tcgataatca acctctggat tacaaaattt
     4201 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4261 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4321 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4381 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4441 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4501 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4561 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4621 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4681 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4741 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     4801 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     4861 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     4921 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4981 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5041 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     5101 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     5161 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     5221 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     5281 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5341 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5401 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5461 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5521 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5581 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5641 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5701 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5761 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6001 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6061 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6121 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6181 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6481 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6541 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6601 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     6661 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     6721 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     6781 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     6841 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     6901 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     6961 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7021 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7081 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7141 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7261 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7321 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7441 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7501 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7561 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7621 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7681 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7741 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7801 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7861 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7921 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7981 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8041 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8101 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8161 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8221 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8281 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8341 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8401 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8461 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8521 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8581 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8641 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8701 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8761 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8821 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8881 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8941 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9001 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9061 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9121 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9181 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9241 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9301 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9361 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9421 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9481 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     9541 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     9601 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     9661 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
//
