LOCUS       sequence-199451-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, 
            Paidemarry S, Green R, Reed RR, Seydoux G
  TITLE     Precision genome editing using synthesis-dependent repair of 
            Cas9-induced DNA breaks.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114.
  PUBMED    29183983
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114."
COMMENT     Sequence Label: pAP1893-1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..48
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 cgtgaccaca tggtccttca tgagtatgta aatgctgctg ggattacagg tggcggccag
       61 cgccgtgcta cgcgaagtgg agctcaagcg gtctctaaag gggaagaact catcaaggag
      121 aatatgcaca tgaagctcta tatggaaggt acagtaaaca atcaccactt caaatgcact
      181 tctgaaggtg aaggaaagcc ctacgagggt acacagacga tgagaattaa agtcgttgag
      241 ggaggcccgc tgccctttgc ttttgacatc cttgcaacct catttatgta tggcagccga
      301 acattcatca atcatacaca ggggattcca gattttttca aacagtcctt tccggagggc
      361 tttacatggg agcgagtaac gacctatgaa gatgggggag ttttgaccgc cacgcaagat
      421 acgtcactcc aagacggctg cctgatttac aatgtaaaaa taagaggcgt taattttccg
      481 tctaatggcc cggtaatgca gaaaaagacc ctgggttggg aggccaacac ggaaatgctt
      541 tatccggcag acggtggctt ggaggggcgc tctgacatgg cactgaagtt ggtcggcgga
      601 ggtcacctta tttgcaactt caaaactacg taccgctcaa agaagccagc caaaaacctt
      661 aaaatgcccg gggtttatta tgtagaccac cgcctcgaac ggatcaagga agctgataaa
      721 gaaacgtatg tagagcaaca tgaagtcgca gtggcaaggt actgtgatct ccccagtaag
      781 ctggggcaca aactgaat
//
