LOCUS       sequence-199454-        1750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human eGFP with homology arms for Lamin A/C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, 
            Paidemarry S, Green R, Reed RR, Seydoux G
  TITLE     Precision genome editing using synthesis-dependent repair of 
            Cas9-induced DNA breaks.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114.
  PUBMED    29183983
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 
            10.1073/pnas.1711979114."
COMMENT     Sequence Label: pAP1716-1
FEATURES             Location/Qualifiers
     source          1..1750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(519..521,522..1232)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(519..521,522..1232)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(561..582)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(822..841)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1169..1190
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctctccctcc ttggctctgc cctctagccc agaaggtctg aggcaatggg ggcaagcttg
       61 gagccgacag tgctgagcag gcaggagcca agagagggga agcttgagcc tcacgcagtt
      121 aggggtgcgc tggagagggt ggggcccgac tccgccacac cccaacggtc cttccccctc
      181 ctcaccactc ccgcccccac ccccaatgga tctgggactg cccctttaag agtagtggcc
      241 cctcctccct tcagaggagg acctattaga gcctttgccc cggcgtcggt gactcagtgt
      301 tcgcgggagc gccgcaccta caccagccaa cccagatccc gaggtccgac agcgcccggc
      361 ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc actccgactc
      421 cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc tgccccgcgg
      481 gcagcgctgc caacctgccg gccatggaga ccccgtccgt gagcaagggc gaggagctgt
      541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1201 ggatcactct cggcatggac gagctgtaca agcagcggcg cgccacccgc agcggggcgc
     1261 aggccagctc cactccgctg tcgcccaccc gcatcacccg gctgcaggag aaggaggacc
     1321 tgcaggagct caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg ctggaaacgg
     1381 agaacgcagg gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc cgcgaggtgt
     1441 ccggcatcaa ggccgcctac gaggccgagc tcggggatgc ccgcaagacc cttgactcag
     1501 tagccaagga gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag gagtttaagg
     1561 agctgaaagc gcggtgagtt cgcccaggtg gctgcgtgcc tggcggggag tggagagggc
     1621 ggcgggccgg cgcccctggc cggccgcagg aagggagtga gagggcctgg aggccgataa
     1681 ctttgccata gtctcctccc tccccggaac tgcccccagc gggtgactgg cagtgtcaag
     1741 gggaattgtc
//
