LOCUS       sequence-199459-        8104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hPMLIII
FEATURES             Location/Qualifiers
     source          1..8104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        47..350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        351..554
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     505..525
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             611..2104
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2114..2116,2117..2119,2120..2830)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2159..2180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2420..2439)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2767..2788
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4878..4999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4915..4934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4969..4988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5006..5461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5143..5164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5355..5374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5488..5592
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5594..5951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5594..5785
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5617..5637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5802..5937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5986..6780
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5986..6780
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6040..6059)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6650..6669
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6968..6987)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7012..7059
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7388..7976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7877..7896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
       61 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      121 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      181 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      241 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      301 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      361 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      421 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      481 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      541 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct
      601 cgaggccacc atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat
      661 tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat
      721 ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa
      781 acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat
      841 caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa
      901 agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga
      961 gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc
     1021 gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa
     1081 atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat
     1141 aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga
     1201 ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc
     1261 tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa
     1321 gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag
     1381 cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa
     1441 cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc
     1501 tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg
     1561 gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg
     1621 ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag
     1681 aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg
     1741 aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg
     1801 gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc
     1861 gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct
     1921 tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa
     1981 agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc
     2041 tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc
     2101 agcagcccgg gtcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2161 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2221 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2281 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     2341 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2401 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2461 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2521 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2581 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2641 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2701 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     2761 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2821 gctgtacaag agcggccgca tggagcctgc acccgcccga tctccgaggc cccagcagga
     2881 ccccgcccgg ccccaggagc ccaccatgcc tccccccgag accccctctg aaggccgcca
     2941 gcccagcccc agccccagcc ctacagagcg agcccccgct tcggaggagg agttccagtt
     3001 tctgcgctgc cagcaatgcc aggcggaagc caagtgcccg aagctgctgc cttgtctgca
     3061 cacgctgtgc tcaggatgcc tggaggcgtc gggcatgcag tgccccatct gccaggcgcc
     3121 ctggccccta ggtgcagaca cacccgccct ggataacgtc tttttcgaga gtctgcagcg
     3181 gcgcctgtcg gtgtaccggc agattgtgga tgcgcaggct gtgtgcaccc gctgcaaaga
     3241 gtcggccgac ttctggtgct ttgagtgcga gcagctcctc tgcgccaagt gcttcgaggc
     3301 acaccagtgg ttcctcaagc acgaggcccg gcccctagca gagctgcgca accagtcggt
     3361 gcgtgagttc ctggacggca cccgcaagac caacaacatc ttctgctcca accccaacca
     3421 ccgcacccct acgctgacca gcatctactg ccgaggatgt tccaagccgc tgtgctgctc
     3481 gtgcgcgctc cttgacagca gccacagtga gctcaagtgc gacatcagcg cagagatcca
     3541 gcagcgacag gaggagctgg acgccatgac gcaggcgctg caggagcagg atagtgcctt
     3601 tggcgcggtt cacgcgcaga tgcacgcggc cgtcggccag ctgggccgcg cgcgtgccga
     3661 gaccgaggag ctgatccgcg agcgcgtgcg ccaggtggta gctcacgtgc gggctcagga
     3721 gcgcgagctg ctggaggctg tggacgcgcg gtaccagcgc gactacgagg agatggccag
     3781 tcggctgggc cgcctggatg ctgtgctgca gcgcatccgc acgggcagcg cgctggtgca
     3841 gaggatgaag tgctacgcct cggaccagga ggtgctggac atgcacggtt tcctgcgcca
     3901 ggcgctctgc cgcctgcgcc aggaggagcc ccagagcctg caagctgccg tgcgcaccga
     3961 tggcttcgac gagttcaagg tgcgcctgca ggacctcagc tcttgcatca cccaggggaa
     4021 agatgcagct gtatccaaga aagccagccc agaggctgcc agcactccca gggaccctat
     4081 tgacgttgac ctgcccgagg aggcagagag agtgaaggcc caggttcagg ccctggggct
     4141 ggctgaagcc cagcctatgg ctgtggtaca gtcagtgccc ggggcacacc ccgtgccagt
     4201 gtacgccttc tccatcaaag gcccttccta tggagaggat gtctccaata caacgacagc
     4261 ccagaagagg aagtgcagcc agacccagtg ccccaggaag gtcatcaaga tggagtctga
     4321 ggaggggaag gaggcaaggt tggctcggag ctccccggag cagcccaggc ccagcacctc
     4381 caaggcagtc tcaccacccc acctggatgg accgcctagc cccaggagcc ccgtcatagg
     4441 aagtgaggtc ttcctgccca acagcaacca cgtggccagt ggcgccgggg aggcagagga
     4501 acgcgttgtg gtgatcagca gctcggaaga ctcagatgcc gaaaactcgg tctcttccag
     4561 ccctcagtct gaggttctgt attggaaagt gcatggagcc catggagacc gccgagccac
     4621 agtcctcgcc agcccactcc tcgccagccc actcctcgcc agcccactcc tcgccagtcc
     4681 agtctctgct gagagcacaa ggagcctcca gcctgccctg tggcacatac caccccccag
     4741 cttggcctcc ccaccagccc gctgacggct agatcataat cagccatacc acatttgtag
     4801 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4861 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4921 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4981 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5041 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5101 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5161 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5221 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5281 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5341 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5401 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5461 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5521 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5581 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5641 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5701 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5761 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5821 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5881 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5941 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6001 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6061 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6121 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6181 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6241 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6301 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6361 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6421 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6481 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6541 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6601 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6661 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6721 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6781 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6841 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6901 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     6961 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7021 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7081 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7141 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7201 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7261 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7321 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7381 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7441 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7501 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7561 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7621 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7681 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7741 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7801 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7861 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7921 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7981 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8041 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     8101 tagt
//
