LOCUS       sequence-199611-        8516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Grefen C, Obrdlik P, Harter K
  TITLE     The determination of protein-protein interactions by the 
            mating-based split-ubiquitin system (mbSUS).
  JOURNAL   Methods Mol Biol. 2009;479:217-33. doi: 
            10.1007/978-1-59745-289-2_14.
  PUBMED    19083185
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "479:217-33. doi"; pages: " 
            10.1007/978-1-59745-289-2_14"
COMMENT     Sequence Label: pXNubA22-Dest
FEATURES             Location/Qualifiers
     source          1..8516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    368..492
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        665..767
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             768..1427
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(834..853)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1769..2074
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1953..1972
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2115..2239)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2385..2474
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2507..2694
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2562..2586)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2721..2737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2721..2737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2734..2756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2745..2761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2769..2775,2776..2793,2794..2799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2814..2835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2952..2969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3123..3711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3203..3222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3882..4673,4674..4742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4505..4524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4743..4847)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4874..6216
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5837..5884)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6257..6275
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6313..6335)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6435..6454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6475..6756
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6757..7431
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7534..7989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7671..7692)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7883..7902
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8115..8137
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8129..8146
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8130..8146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8169..8516,1..358)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactca
      361 gctgtgaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
      421 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      481 ccagtcacta tggcggccgc gggtgatgct gcaacttagc ggccgctaag ttggcagcat
      541 cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa
      601 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
      661 acgttgatcg gcacgcaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      721 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      781 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      841 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      901 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      961 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1021 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1081 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1141 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1201 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1261 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1321 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1381 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
     1441 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     1501 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     1561 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     1621 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     1681 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     1741 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     1801 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     1861 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     1921 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     1981 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     2041 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2101 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     2161 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     2221 ctttcttgta caaagtggtt ggtggtggcg gatccggtgg aggtggatca atgcagattt
     2281 tcgtcaagac tttgaccggt aaaaccgcaa cgttggaagt tgaatcttcc gataccatcg
     2341 acaacgttaa gtcgaaaatt caagacaagg aaggaatccc tggttaccca tacgatgttc
     2401 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
     2461 ttccagatta cgctgctcag tgctgaggcg cgccacttct aaataagcga atttcttatg
     2521 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     2581 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     2641 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagcat
     2701 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3301 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3541 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4861 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     4921 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     4981 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     5041 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     5101 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     5161 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     5221 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     5281 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     5341 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     5401 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     5461 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     5521 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     5581 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     5641 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     5701 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     5761 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     5821 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     5881 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     5941 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     6001 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     6061 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     6121 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     6181 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     6241 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     6301 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     6361 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     6421 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcaacgac
     6481 attactatat atataatata ggaagcattt aatagaacag catcgtaata tatgtgtact
     6541 ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa aatagcttgt
     6601 caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag aattaattcg
     6661 gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga
     6721 gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt
     6781 agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct
     6841 ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt
     6901 gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc
     6961 actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg
     7021 gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac
     7081 caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata
     7141 ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca
     7201 ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag
     7261 agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat
     7321 gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt
     7381 gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact
     7441 gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg
     7501 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
     7561 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7621 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     7681 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     7741 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     7801 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     7861 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     7921 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     7981 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8041 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8101 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctttcgcg
     8161 agctcgatat ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac
     8221 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc
     8281 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta
     8341 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac
     8401 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag
     8461 taataatagg cgcatgcaac ttcttttctt ttttttttct tttctctctc ccccgt
//
