LOCUS       sequence-199634-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Bleymehl K, Perez-Gomez A, Omura M, Moreno-Perez A, Macias D, Bai Z,
            Johnson RS, Leinders-Zufall T, Zufall F, Mombaerts P
  TITLE     A Sensor for Low Environmental Oxygen in the Mouse Main Olfactory 
            Epithelium.
  JOURNAL   Neuron. 2016 Dec 21;92(6):1196-1203. doi: 
            10.1016/j.neuron.2016.11.001. Epub 2016 Dec 1.
  PUBMED    27916458
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.11.001"; journalName: "Neuron"; date: 
            "2016-12-21- 21"; volume: "92"; issue: "6"; pages: "1196-1203"
COMMENT     Sequence Label: pISH-Gucy1b2-A
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(823..841)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(824..841)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(859..875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(859..875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(872..894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    883..899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(907..913,914..931,932..937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cccggccgcc atggcggccg cgggaattcg attcaggcag catggcatac agcaaggtct
       61 ctgtcttctt cttttcaatg gccaggtgat tggaaaggac acgcaactcc tccttcttct
      121 tctccagttg gcaggacagt tccatctctg ccagcctctg ctggttgagg aggatgagat
      181 ccctggtcgt gtcgtgggga gcgatgtcgg aaagatgcat cttgctctcc tccagctctt
      241 gcaggctgcg gagctttgga gaacacatga agaccatgca cttcagagac tccatccaga
      301 tcatctgacc ccggagtttg agtgtcggct ggctcttcca tgcttcgggc atcatctctc
      361 ttcgtgtctt caagataaac tgactgttaa tgaacttgca gatgctggag atgttgaaag
      421 taacttgagg gtggacgatg gagaaatact catctagacc aaacttctgg gtcaagattc
      481 cagggacata cttctgaata ttcactccag cttgcttgac ccttagtgct tcgtcaaaga
      541 caacgtggaa aggaaaagca ttgcagaaca cctcctcttc aatccacagt ctctcaggaa
      601 agacaggctg gaaggtgtcc ccaaggggcc cttttccaag caggactgat gccctcacaa
      661 cagctgagtg agatttctcc ccagggcaaa cagggatgct taaatacctc tccttcttcc
      721 taaggagtgt tccctgaaga gaaatcacta gtgaattcgc ggccgcctgc aggtcgacca
      781 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      841 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      901 tccacacaac atacgagccg gaagcataaa gtgtaaagcc
//
