LOCUS       sequence-199678-       10485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd5
FEATURES             Location/Qualifiers
     source          1..10485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..1258
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          311..1249
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1206..1226
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1278..1302
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3154..3288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3378..3402)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3410..3451
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3502..4090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3555..3575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3973..3984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4162..4395
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4473..4594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4634..4769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4790..4808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4818..4835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4818..4834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4827..4849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4976..5431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5063..5082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5273..5294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5457..5561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5562..5630,5631..6422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5780..5799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6593..7181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7082..7101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7335..7352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7469..7490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7505..7510,7511..7528,7529..7535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7543..7559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7548..7570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7567..7583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7567..7583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7602..7622
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7604..7622
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7650..7876
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7877..8057
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8104..8229
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8722..8955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9140..9184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9415..9625
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9437..9633
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9484..9619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9665..10267
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9665..9684)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10161..10181
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10310..10427
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
       61 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
      121 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
      181 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
      241 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
      301 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
      361 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
      421 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
      481 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
      541 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
      601 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
      661 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
      721 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
      781 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
      841 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
      901 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
      961 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     1021 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     1081 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     1141 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     1201 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     1261 ctagcatcga ttgatcaaca agtttgtaca aaaaagcagg ctggcgccgg aaccaattca
     1321 gtcgactgga tccggtaccg aattcaggcc ggcctaccat ggctcgaccc gacccgtctg
     1381 cgcctccctc tcttctgctg ctgttgctgg cgcagctggt gggccgggca gcggccgcct
     1441 ccaaggcccc ggtgtgccag gaaatcacgg tgcccatgtg ccgaggcatc ggctacaacc
     1501 tgacgcacat gcccaaccag ttcaaccatg acacgcagga cgaagcaggc ctggaggtgc
     1561 accaattctg gccgcttgtg gagatccact gctcaccgga cctgcgcttc ttcctgtgct
     1621 ctatgtacac gcccatctgt ttgcctgact accacaagcc gctaccaccg tgccgttccg
     1681 tgtgcgagcg cgccaaggcc ggctgctcgc cgctcatgcg ccagtacggc ttcgcctggc
     1741 ccgagcgcat gagctgcgac cgcctccctg tgctgggcgg cgacgccgag gttctgtgta
     1801 tggattataa ccgaagcgaa gccaccaccg cgtcccctaa gtccttcccg gccaaaccta
     1861 cactcccagg accaccaggg gcgccatctt ccgggggcga gtgcccctcg ggaggcccat
     1921 ccgtgtgcac gtgccgcgag cccttcgtgc ccatcctgaa ggagtcacac ccactctaca
     1981 acaaggtgcg caccggccaa gtgcccaact gcgcggtgcc ctgctaccag ccgtccttca
     2041 gcccggacga gcgcacgttc gccaccttct ggattggcct gtggtctgtg ctgtgcttca
     2101 tctccacgtc caccaccgtt gccaccttcc tcattgacat ggaacgattc cgctaccctg
     2161 agcgccccat catcttcttg tctgcgtgct acctgtgtgt gtcactggga ttcttggtgc
     2221 gcctggtagt gggccatgcc agcgtcgctt gcagccgtga gcacagccac attcactatg
     2281 agactaccgg ccctgcgctg tgcacggttg tcttcctctt agtctatttc tttggcatgg
     2341 ccagctccat ctggtgggtc atcctgtcgc tcacctggtt cttggcggct ggcatgaagt
     2401 ggggcaatga agccatcgca ggttatgcac agtacttcca ccttgctgcc tggctcatcc
     2461 ccagtgtcaa gtccattacg gcgctggcac tgagctcggt ggacggggac ccagtggctg
     2521 gcatctgcta tgtgggcaac caaaacctga actcactacg aggctttgtc ttgggcccac
     2581 tggtgctgta cctgttggtg ggcacgctct tccttctggc aggcttcgtg tcactcttcc
     2641 gcatccggag cgtcatcaag cagggtggca ctaagacgga caagctagag aagctcatga
     2701 tccgcatcgg catcttcacc ctgctctaca cggtgccagc cagcatcgtg gtggcctgct
     2761 acctgtatga gcagcactac cgggagagct gggaggcagc cctcacctgc gcgtgtccgg
     2821 gaccggacgc tggccagcca cgcgccaaac ccgagtactg ggtgctcatg ctcaagtact
     2881 tcatgtgcct ggtggtgggc atcacgtcgg gagtctggat ctggtccggc aagactctgg
     2941 agtcttggcg gcggttcacc agccgctgct gctgcagctc tcggcggggc cacaagagcg
     3001 gtggcgctat ggccgcagga gactatgcgg aggccagcgc cgcgctcacc ggcaggaccg
     3061 ggccgcctgg ccccaccgcc gcataccaca agcaagtgtc cctgtcgcac gtatagggcg
     3121 cgcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     3181 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3241 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3301 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     3361 gcactcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcg gtaagcctat
     3421 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtaccg gttaagtcga
     3481 caatcaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3541 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3601 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3661 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3721 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3781 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3841 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3901 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3961 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4021 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4081 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4141 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4201 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4381 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4441 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4741 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4801 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5401 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6721 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7381 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7441 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7501 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7561 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7621 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7681 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7741 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7801 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7861 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7921 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7981 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8041 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8101 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8161 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8221 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8281 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8341 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8401 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8461 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8521 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8581 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8641 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8701 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8761 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8821 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8881 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8941 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9001 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9061 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9121 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9181 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9241 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9301 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9361 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9421 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9601 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9661 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9721 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9781 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9841 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9901 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9961 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
    10021 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
    10081 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
    10141 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    10201 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    10261 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    10321 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10381 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10441 gggacagcag agatccactt tggcgccggc tcgaggcctg caggt
//
