LOCUS       sequence-199679-       10857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd6
FEATURES             Location/Qualifiers
     source          1..10857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        91..1269
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          322..1260
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1217..1237
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1289..1313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3537..3671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3761..3785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3793..3834
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3885..4473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3938..3958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4356..4367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4545..4778
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4856..4977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5017..5152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5173..5191)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5201..5218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5201..5217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5210..5232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5359..5814
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5446..5465)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5656..5677
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5840..5944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5945..6013,6014..6805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6163..6182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6976..7564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7465..7484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7718..7735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7852..7873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7888..7893,7894..7911,7912..7918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7926..7942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7931..7953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7950..7966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7950..7966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7985..8005
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7987..8005
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8033..8259
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8260..8440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8487..8612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9105..9338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9523..9567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9798..10008
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9820..10016
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9867..10002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10048..10650
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10048..10067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10544..10564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10693..10810
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
       61 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      121 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      181 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      241 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      301 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      361 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      421 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      481 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      541 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      601 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      661 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      721 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      781 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
      841 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
      901 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
      961 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1021 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1081 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1141 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1201 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1261 gtgtcgtgag gctagcatcg attgatcaac aagtttgtac aaaaaagcag gctggcgccg
     1321 gaaccaattc agtcgactgg atccggtacc gaattcaggc cggcctacca tggaaaggtc
     1381 cccgtttctg ttggcgtgca ttcttctgcc cctcgtaaga ggacacagcc ttttcacctg
     1441 tgagccaatc accgttccca gatgtatgaa aatgacttac aacatgacgt tcttccctaa
     1501 cctgatgggt cattatgacc aggggatcgc tgctgtggaa atggggcact ttctgcatct
     1561 tgcaaatcta gaatgttcac caaacattga aatgttcctt tgccaagctt ttataccaac
     1621 ctgcacagag caaattcatg tagttctacc ctgtcggaaa ttgtgtgaga aaatagtttc
     1681 tgattgcaaa aaactaatgg acacttttgg catccgatgg cctgaagaac ttgaatgtaa
     1741 cagattgcca cactgtgatg acactgttcc tgtaacttct catccacaca cagagctttc
     1801 tgggccacag aagaaatcag atcaagtccc aagagacatt ggattttggt gtccaaagca
     1861 ccttaggact tccggggacc aaggctatag gtttctggga attgaacagt gtgcccctcc
     1921 gtgccccaat atgtatttta aaagtgatga actagacttt gccaaaagtt tcataggaat
     1981 agtttcaata ttttgtcttt gtgcaactct gttcacgttc cttacatttt taattgacgt
     2041 tagacgattc agatacccag agagaccaat tatctattac tctgtctgct acagcattgt
     2101 ctctctcatg tacttcgtgg ggtttttgct gggcaatagc acggcttgta ataaggcaga
     2161 cgagaagctg gagctcgggg acaccgttgt cctagggtca aagaataagg cttgcagtgt
     2221 ggtatttatg tttctgtatt tttttacaat ggctggcacc gtgtggtggg tgattctcac
     2281 cattacgtgg ttcttagctg ccgggagaaa atggagttgc gaagctattg aacaaaaagc
     2341 agtgtggttc catgccgttg cctggggggc gcccgggttc ctgaccgtca tgctgctcgc
     2401 tatgaataag gttgaaggag acaacattag cggcgtttgc ttcgttggcc tgtatgacct
     2461 ggacgcctct cgctacttcg tccttctgcc tctgtgcctc tgcgtatttg ttgggctgtc
     2521 tctcctctta gccggcatca tctccttgaa tcatgtccga caagtcatac agcatgatgg
     2581 ccggaaccaa gagaagctaa agaaattcat gattcgcatc ggagtcttca gtggcctgta
     2641 tcttgtgccc ttagtgacac ttctcggttg ctatgtctat gagctagtga acaggatcac
     2701 ctgggagatg acatggttct ctgatcattg tcaccagtac cgcatcccgt gcccttacca
     2761 ggcaaatcca aaagctcgac cagaattggc tttatttatg ataaaatatc tgatgacatt
     2821 aattgttggt atctctgcgg tcttctgggt tggaagcaaa aagacgtgca cagaatgggc
     2881 cgggttcttt aagcgaaacc gcaagcgaga ccccatcagt gagagccgcc gagtgctgca
     2941 agagtcctgt gagttcttcc tgaagcacaa ctctaaagtg aagcacaaga agaagcatgg
     3001 cgcaccaggg cctcataggc tgaaggtcat ttccaagtcc atgggaacta gcacaggagc
     3061 gaccacaaat catggcacct ctgccatggc aatcgctgac catgattact tagggcaaga
     3121 aacttcaaca gaagtccaca cctccccaga agcatccgtc aaagagggac gagcagaccg
     3181 agcaaacact cccagcgcca aagatcggga ctgtggggaa tctgcagggc ccagttccaa
     3241 gctctctggg aaccggaacg gcagggaaag ccgagcgggc ggcctgaagg agagaagcaa
     3301 tggatcagag ggggctccaa gtgaaggaag ggtaagtcca aagagcagcg ttcctgagac
     3361 tggcctgata gactgcagca cttcacaggc cgccagttct ccagaaccaa ccagcctcaa
     3421 gggctccaca tctctgcctg ttcactcagc ttccagagct aggaaagagc agggtgctgg
     3481 cagccattcc gacgcttgag gcgcgcctct agaactatag tgagtcgtat tacgtagatc
     3541 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3601 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3661 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3721 gggaggtttt ttaattcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa
     3781 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     3841 tgaactagta ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa cctctggatt
     3901 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3961 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4021 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4081 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4141 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4201 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4261 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4321 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4381 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4441 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     4501 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4561 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     4801 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     4861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4981 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     5161 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     5221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5341 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5701 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5761 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5821 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5881 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5941 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6001 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6061 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6121 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6181 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6241 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6301 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6361 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6421 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6481 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6541 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6601 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6661 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6721 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6781 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6841 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6901 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7081 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7621 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7681 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7741 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7801 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7861 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7921 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7981 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     8041 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     8101 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     8161 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     8221 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     8281 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8341 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8401 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8461 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8521 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8581 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8641 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8701 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8761 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8821 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8881 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8941 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9001 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9061 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9121 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9181 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9241 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9301 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9361 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9421 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9481 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9541 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9601 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     9661 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9721 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa
     9781 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa
     9841 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9901 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9961 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10021 tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
    10081 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
    10141 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
    10201 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
    10261 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
    10321 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
    10381 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
    10441 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
    10501 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
    10561 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
    10621 tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt
    10681 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    10741 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    10801 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcga
//
