LOCUS       sequence-199841-        2208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ODPBMTS in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD2-ODPBMTS (12)
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      228..285
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(309..326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(480..1068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(560..579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1251..1345)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1366..2025)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1940..1959
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2187..2205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagcg atgagtggtc tcgccatgtt ttccagactg ccaacatcat tggccagaaa
      121 tgttgcacgt cgtgccccaa cttcttttgt aagaccctct gcagcagcag cagcattgag
      181 attctcaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag
      241 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
      301 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      481 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1201 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1261 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1321 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1381 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1441 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1501 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1561 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt
     1621 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1681 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg
     1741 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1801 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1861 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1921 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1981 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2041 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2101 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     2161 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcaga
//
