LOCUS       sequence-199852-        1263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant bacterial expression of SnoopLigase, a 
            protein that promotes the formation of an isopeptide bond between 2 
            peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Buldun CM, Jean JX, Bedford MR, Howarth M
  TITLE     SnoopLigase Catalyzes Peptide-Peptide Locking and Enables 
            Solid-Phase Conjugate Isolation.
  JOURNAL   J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 
            10.1021/jacs.7b13237. Epub 2018 Feb 14.
  PUBMED    29402082
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.7b13237"; journalName: "J Am Chem Soc"; date: 
            "2018-02-28- 28"; volume: "140"; issue: "8"; pages: "3008-3018"
COMMENT     Sequence Label: pET28a-HaloTag7-SnoopLigase
FEATURES             Location/Qualifiers
     source          1..1263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..924
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg aaatcggtac tggctttcca
       61 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
      121 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
      181 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg
      241 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
      301 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
      361 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
      421 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
      481 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
      541 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
      601 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
      661 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
      721 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
      781 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg
      841 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
      901 ctgtctactc tggagatttc cggtggtagc ggtgggtctg gtagtggtgt gaataagaac
      961 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc
     1021 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat
     1081 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa
     1141 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc
     1201 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc
     1261 taa
//
