LOCUS       sequence-19987-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K
  TITLE     Vectors for co-expression of an unrestricted number of proteins.
  JOURNAL   Nucleic Acids Res. 2007;35(6):e43. doi: 10.1093/nar/gkm067. Epub 
            2007 Feb 20.
  PUBMED    17311810
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35(6):e43. doi"; pages: " 
            10.1093/nar/gkm067. Epub 2007 Feb 20"
COMMENT     Sequence Label: pQLinkG2
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             45..65
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(103..127)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      149..243
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(403..422)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggatctggg tgctgcaggt acaagtttgt acaaaaaagc aggcgagaat ctttattttc
       61 agggatccag tcttcgcatg catgaagacg cggccgccta ggacccagct ttcttgtaca
      121 aagtggtaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      181 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      241 aatccaagta acaacaccat ttaaatggag tggttacaaa tggagtggtt aattaaggct
      301 agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      361 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      421 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      481 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      541 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      601 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      661 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      721 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
      781 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatgg
//
