LOCUS       sequence-199895-        1333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fragment (amnio acids 328-354) of human Champ1 protein 
            tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Thanos Halazonetis
  TITLE     Expression plasmids for production of Rev7 and Champ1 proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIRESN2_GFP_YTA_Champ1_328-354
FEATURES             Location/Qualifiers
     source          1..1333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..119
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     70..90
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     164..183
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        164..182
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      297..306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(342..370)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     965..985
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
       61 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      121 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      181 gggagaccca agcttggtac cgagctcgga tcgatatctg cggcctagct agcgctaccg
      241 gactcagatc tcgagctcaa gcttcgaatt ctaagtgagt aaaggaccca ccggtcgcca
      301 ccatggaatc ctctagcaaa ggagaagaac tcttcactgg agttgtccca attcttgttg
      361 aattagatgg tgatgttaac ggccacaagt tctctgtcag tggagagggt gaaggtgatg
      421 caacatacgg aaaacttacc ctgaagttca tctgcactac tggcaaactg cctgttcctt
      481 ggccaacact agtcactact ctgtgctatg gtgttcaatg cttttcaaga tacccggatc
      541 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgta
      601 ccatctactt caaagatgac ggcaactaca agacacgtgc tgaagtcaag tttgaaggtg
      661 atacccttgt taatagaatc gagttaaaag gtattgactt caaggaagat ggcaacattc
      721 tgggacacaa actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac
      781 aaaagaatgg gatcaaagct aacttcaaga cccgccacaa cattgaagat ggaagcgttc
      841 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      901 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      961 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactgt
     1021 ataagggatc ctcagcatca tcaggacctt ggaagccagc taaacctgct ccatctgtgt
     1081 ctcctggacc ttggaaacca attccttctg tatgagcggc cgcatagata actgatccag
     1141 tgtgctggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctca
     1201 aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata
     1261 ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca
     1321 atctttttgt tgt
//
