LOCUS       sequence-200042-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd1
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..1264
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          317..1255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1212..1232
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1284..1308
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3331..3465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3555..3579)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3587..3628
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3679..4267
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3732..3752)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4150..4161)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4339..4572
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4650..4771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4811..4946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4967..4985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4995..5012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4995..5011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5004..5026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5153..5608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5240..5259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5450..5471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5634..5738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5739..5807,5808..6599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5957..5976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6770..7358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7259..7278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7512..7529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7646..7667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7682..7687,7688..7705,7706..7712)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7720..7736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7725..7747
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7744..7760
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7744..7760
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7779..7799
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7781..7799
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7827..8053
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8054..8234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8281..8406
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8899..9132
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9317..9361
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9592..9802
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9614..9810
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9661..9796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9842..10444
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9842..9861)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10338..10358
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10487..10604
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
       61 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
      121 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      181 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      241 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      301 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      361 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg cgccggaacc
     1321 aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggct gaggaggcgg
     1381 cgcctagcga gtcccgggcc gccggccggc tgagcttgga actttgtgcc gaagcactcc
     1441 cgggccggcg ggaggaggtg gggcacgagg acacggccag ccaccgccgc ccccgggctg
     1501 atccccggcg ttgggctagc gggctgctgc tgctgctttg gttgctggag gctcctctgc
     1561 ttttgggggt ccgagcgcag gcggcgggcc aggtatccgg gccgggccag caagccccgc
     1621 cgccgcccca gccccagcag agcgggcagc agtacaacgg cgaacggggc atctccatcc
     1681 cggaccacgg ctactgccag cccatctcca tcccgctgtg cacggacatc gcgtacaacc
     1741 agaccatcat gcccaacctg ctgggccaca cgaatcagga ggacgccggt ctggaggtgc
     1801 accagttcta ccctctggtg aaggtgcagt gctccgccga gctcaagttc ttcctgtgct
     1861 ccatgtacgc gcctgtgtgc accgtactgg agcaggcgct accgccctgc cgctccctgt
     1921 gcgagcgcgc acgccagggc tgcgaggcgc tcatgaacaa gttcggcttc cagtggccag
     1981 acacactcaa gtgcgagaag ttcccggtgc acggcgcagg agagctgtgc gtgggccaga
     2041 acacgtccga caaaggcacc ccaactccct ccttgctacc agagttctgg accagtaatc
     2101 cgcagcacgg cggcggtggt taccgcggcg gctacccggg gggtgccggg acggtggagc
     2161 ggggaaagtt ctcctgcccg cgcgccctca gggtgccctc ctacctcaac taccactttc
     2221 tgggggagaa ggactgcggc gcaccctgcg aacccaccaa ggtttacggg ctcatgtact
     2281 tcgggccaga ggagctgcgc ttctcgcgca cctggatagg catctggtcc gtgctgtgct
     2341 gcgcctccac gctcttcacg gtgctcacgt acctagtgga catgcggcgc ttcagctacc
     2401 cggaacggcc catcattttc ctgtccggct gttacacagc ggtggcggtg gcctacatcg
     2461 ctggctttct gttggaggac cgggtggtgt gcaacgacaa gtttgcagag gacggggcgc
     2521 gcacggtggc gcagggcact aagaaagaag gctgcactat actctttatg atgctctact
     2581 tcttcagcat ggccagctcc atctggtggg tgatcctgtc cctcacctgg ttcctggcag
     2641 ccggcatgaa gtggggccac gaagccatcg aggccaactc acagtatttc catttagccg
     2701 cctgggctgt gccagccatc aaaactataa ccatcttggc gttgggccag gtggatggcg
     2761 acgtactgag cggagtgtgt tttgtggggc tcaacaacgt ggacgcactg cgtggctttg
     2821 tgctggcgcc tctcttcgtt tatctgttca ttggcacttc tttcctgctg gccggtttcg
     2881 tgtcactctt ccgcatccgc accatcatga agcatgacgg caccaagaca gagaagctgg
     2941 agaagctcat ggtgcgcatc ggagtcttca gtgtcctcta cactgtgccg gccaccatcg
     3001 tcatcgcctg ctacttctat gaacaggcct ttcgggacca gtgggagcgc agctgggtgg
     3061 cccagagctg caagagttat gccatccctt gccctcacct ccagggaggt ggaggagtcc
     3121 caccacaccc gcccatgagc ccagacttta cagtcttcat gatcaagtat ctcatgacgc
     3181 tgattgtggg catcacatcg ggcttctgga tctggtccgg caagacactg aattcctgga
     3241 ggaagttcta cacgaggctt accaacagca aacaggggga gactaccgtc tgaggcgcgc
     3301 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     3361 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3421 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3481 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgca
     3541 ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatcggta agcctatccc
     3601 taaccctctc ctcggtctcg attctacgta gtaatgaact agtaccggtt aagtcgacaa
     3661 tcaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4081 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4261 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4321 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4381 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4441 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4501 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4561 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4621 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4681 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4861 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4921 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4981 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5581 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5641 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5701 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5761 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5821 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5881 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5941 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6001 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6061 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6121 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6181 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6241 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6301 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6361 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6421 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6481 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6541 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6601 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6661 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6721 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6901 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7141 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7381 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7441 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7501 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7561 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7621 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7681 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7741 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7801 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7861 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7921 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7981 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8041 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8101 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8161 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8221 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8281 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8341 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8401 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8461 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8521 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8581 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8641 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8701 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8761 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8821 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8881 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8941 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9001 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9061 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9121 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9181 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9241 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9301 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9361 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9421 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9481 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9541 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9601 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9661 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9721 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9781 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9841 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9901 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9961 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10021 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    10081 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10141 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10201 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10261 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10321 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10381 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    10441 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10501 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10561 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10621 acagcagaga tccactttgg cgccggctcg aggcct
//
