LOCUS       sequence-20007-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Jones DL, Alani RM, Munger K
  TITLE     The human papillomavirus E7 oncoprotein can uncouple cellular 
            differentiation and proliferation in human keratinocytes by 
            abrogating p21Cip1-mediated inhibition of cdk2.
  JOURNAL   Genes Dev. 1997 Aug 15. 11(16):2101-11.
  PUBMED    9284049
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1997 Aug 15. 11(16):2101-11."
COMMENT     Sequence Label: pGEX2TK E7 DLYC
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(391..413)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(558..576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gap             704..715
                     /estimated_length=12
ORIGIN
        1 cggatctggt tccgcgtgga tctcgtcgtg catctgttgg atccatgcat ggagatacac
       61 ctacattgca tgaatatatg ttagatttgc aaccagagac aacatatgag caattaaatg
      121 acagctcaga ggaggaggat gaaatagatg gtccggccgg acaagcagaa ccggacagag
      181 cccattacaa tattgtaacc ttttgttgca agtgtgactc tacgcttcgg ttgtgcgtac
      241 aaagcacaca cgtagacatt cgtactttgg aagacctgtt aatgggcaca ctaggaattg
      301 tgtgccccat ctgttctcag aaaccataag atgaattcat cgtgactgac tgacgatctg
      361 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      421 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
      481 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa
      541 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      601 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      661 tttatttttc taaatacatt caaatatgta tccgctcatg agannnnnnn nnnnnantnt
      721 anntntttnn nntattttna aaaaanaaaa aatttttact ttctctcnnn cnnnccnggg
      781 nccncccctt nttttcccct ttttttgccg gcaa
//
