LOCUS       sequence-200206-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Mbefo MK, Fares MB, Paleologou K, Oueslati A, Yin G, Tenreiro S, 
            Pinto M, Outeiro T, Zweckstetter M, Masliah E, Lashuel HA
  TITLE     Parkinson disease mutant E46K enhances alpha-synuclein 
            phosphorylation in mammalian cell lines, in yeast, and in vivo.
  JOURNAL   J Biol Chem. 2015 Apr 10;290(15):9412-27. doi: 
            10.1074/jbc.M114.610774. Epub 2015 Feb 5.
  PUBMED    25657004
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.610774"; journalName: "J Biol Chem"; date: 
            "2015-04-10- 10"; volume: "290"; issue: "15"; pages: "9412-27"
COMMENT     Sequence Label: pcDNA6 asyn E46K
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(529..553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(600..617)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    606..831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(964..983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttact ttagaaggag atatacatat ggatgtattc atgaaaggac tttcaaaggc
       61 caaggaggga gttgtggctg ctgctgagaa aaccaaacag ggtgtggcag aagcagcagg
      121 aaagacaaaa gagggtgttc tctatgtagg ctccaaaacc aagaagggag tggtgcatgg
      181 tgtggcaaca gtggctgaga agaccaaaga gcaagtgaca aatgttggag gagcagtggt
      241 gacgggtgtg acagcagtag cccagaagac agtggaggga gcagggagca ttgcagcagc
      301 cactggcttt gtcaaaaagg accagttggg caagaatgaa gaaggagccc cacaggaagg
      361 aattctggaa gatatgcctg tggatcctga caatgaggct tatgaaatgc cttctgagga
      421 agggtatcaa gactacgaac ctgaagccta agaaatatct ttgctcccag tttcttgaga
      481 tctgctgaca gatgttccat ccaagcttcg agaagggtgg gcgcgccgac ccagctttct
      541 tgtacaaagt ggtgataatt aattaagatc tagagggccc gtttaaaccc gctgatcagc
      601 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      661 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      721 ttgtctgagt aggtgtcatt ctattctggg gggtggnnnt ggggcaggac agcaaggggg
      781 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      841 cgganagaac cagctggggc tctagggggt atccncacgc gccctgtagc ggcgcattaa
      901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1021 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctc
//
