LOCUS       sequence-200218-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn 1-106AA Mxe Histag
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(214..231)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(256..849)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aattctcatg
       61 tttgacagct tatcatcgat aagcttggat ggaacatctg tcagcagatc tcaagaaact
      121 gggagcaaag atatttctta ggcttcaggt tcgtagtctt gatacccttc ctcagaaggc
      181 atttcataag cctcattgtc aggatcctta tcagtggtgg tggtggtggt ggaattccag
      241 accggtgagg ccagtagcgt ggctgacgaa cccgttcgtg ataaacgcgt ggtctgccgt
      301 gtcgacacga aggctataca ccggctgcac gccggcgtcg gtgacactgg cgactttcgc
      361 gtagtagaac cgcccgtcgg tcagctcgtc ggcgatagct tgggcgtccg ggtctcggtg
      421 gtgtgcttcc aagaaacgca ccagtccagg gacgccgact gtgtaggttg tgggcgcaaa
      481 ttcgggtttc ccgcgggcaa aacctgcaca gtcgacgctg aatgcgctgc gttgaatcac
      541 cgcgtaatcg cccggcttga tttcgtcgat cagcttccac agcagggtcg gcaccccggc
      601 gacgtcgacc aaacacaaca acgggtggtt cgcggtgccc gtcacacgca gaccttcgac
      661 cgtacgcacc gtgtacaccg gatgctcgcc ggagtggaac agccggtcgg cgagcacggg
      721 attgccatgc cggtcaagga ctttcaggtc gatggcgttg tcactgttgg gccgcgcacc
      781 cggcacgatg tcggcgatgc gtaccgactc gccctcgggt agggcaacta gtgcatctcc
      841 cgtgatgcat ccttcttcat tcttgcccaa ctggtccttt ttgacaaagc cagtggctgc
      901 tgcaatgctc cctgctccct ccactgtctt ctgg
//
