LOCUS       sequence-200260-        9245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing 
            SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, 
            Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L,
            Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch
            R, Gregory PD, Urnov FD
  TITLE     Functional genomics, proteomics, and regulatory DNA analysis in 
            isogenic settings using zinc finger nuclease-driven transgenesis 
            into a safe harbor locus in the human genome.
  JOURNAL   Genome Res. 2010 Aug;20(8):1133-42. doi: 10.1101/gr.106773.110. Epub
            2010 May 27.
  PUBMED    20508142
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.106773.110"; journalName: "Genome Res"; date: "2010-08";
            volume: "20"; issue: "8"; pages: "1133-42"
COMMENT     Sequence Label: AAVS1-Neo-M2rtTA
FEATURES             Location/Qualifiers
     source          1..9245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             961..1764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1774..2235
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        2333..2636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3075..3097
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3159..3178
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(3251..3868,3869..3874,3875..3910,3911..3913,3914..3949,
                     3950..3952,3953..3988,3989..3997)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    4007..4141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4031..4050)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4085..4104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    4481..5317
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5351..5370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5352..5370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5377..5394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5377..5393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5386..5408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5531..5833
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5712..5731
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(7230..8021,8022..8090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7853..7872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8214..8802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8703..8722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8956..8973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9090..9111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9126..9131,9132..9149,9150..9156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9164..9180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9169..9191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9188..9204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9188..9204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9225..9243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagacgcgt
      961 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1021 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1081 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1141 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1201 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1261 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1321 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1381 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1441 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1501 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1561 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1621 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1681 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1741 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     1801 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
     1861 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
     1921 ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa
     1981 tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt
     2041 cctcccactc atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga
     2101 ttcccacttt gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag
     2161 aacgagatca gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt
     2221 ctaattccat cagaagctgg gagctccacc gcggtggcgg ccgctctaga actagttatt
     2281 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2341 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2401 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2461 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2521 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2581 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     2641 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     2701 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggagggctc
     2761 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     2821 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     2881 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     2941 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     3001 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     3061 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     3121 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3181 gtctcatcat tttggcaaag aattcggctt gatcgaagct agtggatccc ccgggctgca
     3241 ggaattcacc atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact
     3301 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     3361 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     3421 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     3481 ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc
     3541 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     3601 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     3661 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     3721 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     3781 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     3841 ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt
     3901 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     3961 tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc cagacatgat
     4021 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4081 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4141 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4201 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctg caagcctcgt
     4261 cgtcctggcc ggaccacgct atctgtgcaa ggtccccggc cccggacgcg cgctccatga
     4321 gcagagcgcc cgccgccgag gcgaagactc gggcggcgcc ctgcccgtcc caccaggtca
     4381 acaggcggta accggcctct tcatcgggaa tgcgcgcgac cttcagcatc gccggcatgt
     4441 ccccctggcg gacgggaagt atccagctcg accaagcttt actagggaca ggattggtga
     4501 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
     4561 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
     4621 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
     4681 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
     4741 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
     4801 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
     4861 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
     4921 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
     4981 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
     5041 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
     5101 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
     5161 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
     5221 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
     5281 cctgcccctt ccctacaggg gttcctggct ctgctctaag ggcgaattcg cggccgctaa
     5341 attcaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     5401 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5461 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5521 atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     5581 ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag
     5641 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     5701 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     5761 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     5821 ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt
     5881 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     5941 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     6001 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     6061 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     6121 aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg
     6181 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6241 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6301 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6361 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6421 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6481 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6541 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6601 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6661 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     6721 cggcgaggat ctcgtcgtga cccatgcatg gcgatgcctg cttgccgaat atcatggtgg
     6781 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6841 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6901 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6961 ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc
     7021 ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc
     7081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagatta
     7141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7741 actgtcatgc catccgtaag atgcttttct gtgactggtg aatactcaac caagtcattc
     7801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgaccaaaat cccttaacgt
     8161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9061 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9181 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcagaattaa ccctcactaa
     9241 aggga
//
