LOCUS       sequence-20027-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Baldwin A, Huh KW, Munger K
  TITLE     Human papillomavirus E7 oncoprotein dysregulates steroid receptor 
            coactivator 1 localization and function.
  JOURNAL   J Virol. 2006 Jul . 80(13):6669-77.
  PUBMED    16775354
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 Jul . 80(13):6669-77."
COMMENT     Sequence Label: cmv 16 E7 Flag/HA
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             478..504
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             478..504
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(603..622)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt ctctttccta
       61 cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattcctc
      121 gacggatcca tgcatggaga tacacctaca ttgcatgaat atatgttaga tttgcaacca
      181 gagacaactg atctctactg ttatgagcaa ttaaatgaca gctcagagga ggaggatgaa
      241 atagatggtc cagctggaca agcagaaccg gacagagccc attacaatat tgtaaccttt
      301 tgttgcaagt gtgactctac gcttcggttg tgcgtacaaa gcacacacgt agacattcgt
      361 actttggaag acctgttaat gggcacacta ggaattgtgt gccccatctg ttctcagaaa
      421 ccagcggccg ctggaggaga ctacaaggac gacgatgaca agtcggccgc tggaggatac
      481 ccctacgacg tgcccgacta cgcctaggga tccgtcgagg aattcactcc tcaggtgcag
      541 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
      601 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
      661 tctggctaat aaaggaaatt tattttcatt gcaatagtgt
//
