LOCUS       sequence-20035-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: SIRT4.23
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(40..54,55..60,61..77,78..84)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    92..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..138
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aggcgtatca cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
       61 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
      121 ttcattaaag aggagaaatt aactatgaga ggatcgcatc accatcacca tcacggatcc
      181 atgaagatga gctttgcgtt gactttcagg tcagcaaaag gccgttggat cgcaaacccc
      241 agccagccgt gctcgaaagc ctccattggg ttatttgtgc cagcaagtcc tcctctggac
      301 cctgagaagg tcaaagagtt acagcgcttc atcacccttt ccaagagact ccttgtgatg
      361 actggggcag gaatctccac cgaatcgggg ataccagact acaggtcaga aaaagtgggg
      421 ctttatgccc gcactgaccg caggcccatc cagcatggtg attttgtccg gagtgcccca
      481 atccgccagc ggtactgggc gagaaacttc gtaggctggc ctcaattctc ctcccaccag
      541 cctaaccctg cacactgggc tttgagcacc tgggagaaac tcggaaagct gtactggttg
      601 gtgacccaaa atgtggatgc tttgcacacc aaggcgggga gtcggcgcct gacagagctc
      661 cacggatgca tggacagggt cctgtgcttg gattgtgggg aacagactcc ccggggggtg
      721 c
//
