LOCUS       sequence-200464-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding comTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-comTurquoise2
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..28
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      967..1053
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1591..2541)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2589..2811
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3303..3397)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3428..4222)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3539..3558)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4149..4168
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 acctataaaa ataggcgtat cacgaggccc tttcgtcttc accacagcta acaccacgtc
       61 gtccctatct gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata
      121 aggctcgtat gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt
      181 aacttttcta gatttaagaa ggagatatac atatgagcaa gggcgaggag ctgtttactg
      241 gtgtggttcc tatcctggtc gaactggacg gtgatgtcaa cggtcataag ttcagcgtta
      301 gcggcgaggg tgaaggcgac gcaacctacg gtaagctgac cctgaagttc atttgtacga
      361 cgggtaagct gccggtgcct tggcctaccc tggttaccac cctgagttgg ggtgttcaat
      421 gcttcgctcg ctatccagac cacatgaagc agcacgattt ttttaagagc gccatgccag
      481 agggttacgt tcaggagcgc accattttct ttaaagatga tggtaattac aagactcgcg
      541 cagaggtgaa gtttgaaggc gacactctgg tgaatcgtat tgagctgaag ggcatcgatt
      601 ttaaggaaga tggtaatatc ctgggccata aactggaata taattacttc agcgataatg
      661 tgtatatcac cgccgataaa caaaagaatg gtattaaggc gaattttaaa attcgccata
      721 atatcgagga cggcggcgtg caactggctg accattatca acaaaatacc ccgattggcg
      781 acggtcctgt tctgctgccg gacaatcact atctgagcac tcaatctaag ctgagcaaag
      841 atcctaatga gaagcgcgat cacatggtgc tgctggaatt tgtaacggct gctggcatca
      901 cgcatggcat ggatgagctg tataaataaa tgtccagacc tgcaggcatg caagctctag
      961 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1021 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta gggtacgggt
     1081 tttgctgccc gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc
     1141 ggcttcagcc ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc
     1201 cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc
     1261 gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca
     1321 atttcatgtt ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc
     1381 tgttcatctg ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact
     1441 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt
     1501 tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     1561 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     1621 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
     1681 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     1741 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
     1801 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     1861 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     1921 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     1981 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2041 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2101 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2161 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2221 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     2281 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     2341 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     2401 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     2461 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     2521 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     2581 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
     2641 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     2701 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     2761 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     2821 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     2881 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     2941 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3001 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     3061 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     3121 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
     3181 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
     3241 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt
     3301 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     3361 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga
     3421 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     3481 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     3541 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     3601 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     3661 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
     3721 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     3781 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     3841 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     3901 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     3961 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     4021 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     4081 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     4141 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     4201 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
     4261 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
     4321 tcccaacctt accagagggc gccccagctg gcaattccga cgtctaagaa accattatta
     4381 tcatgacatt a
//
