LOCUS       sequence-200466-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding coSCFP3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-coSCFP3A
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      996..1082
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1620..2570)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2618..2840
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3332..3426)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3457..4251)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3568..3587)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4178..4197
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
       61 ttcgtcttca ccacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg
      121 gttgctggat aactttacgg gcatgcataa ggctcgtatg atatattcag ggagaccaca
      181 acggtttccc tctacaaata attttgttta acttttctag atttaagaag gagatataca
      241 tatgagcaag ggcgaggagc tgtttactgg tgtggttcct atcctggtcg aactggacgg
      301 tgatgtcaac ggtcataagt tcagcgttag cggcgagggt gaaggcgacg caacctacgg
      361 taagctgacc ctgaagttca tttgtacgac gggtaagctg ccggtgcctt ggcctaccct
      421 ggttaccacc ctgacctggg gtgttcaatg cttcgctcgc tatccagacc acatgaagca
      481 gcacgatttt tttaagagcg ccatgccaga gggttacgtt caggagcgca ccattttctt
      541 taaagatgat ggtaattaca agactcgcgc agaggtgaag tttgaaggcg acactctggt
      601 gaatcgtatt gagctgaagg gcatcgattt taaggaagat ggtaatatcc tgggccataa
      661 actggaatat aattacatta gcgataatgt gtatatcacc gccgataaac aaaagaatgg
      721 tattaaggcg aattttaaaa ttcgccataa tatcgaggac ggcggcgtgc aactggctga
      781 ccattatcaa caaaataccc cgattggcga cggtcctgtt ctgctgccgg acaatcacta
      841 tctgagcact caatctaagc tgagcaaaga tcctaatgag aagcgcgatc acatggtgct
      901 gctggaattt gtaacggctg ctggcatcac gcatggcatg gatgagctgt ataaataaat
      961 gtccagacct gcaggcatgc aagctctaga ggcatcaaat aaaacgaaag gctcagtcga
     1021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     1081 atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt
     1141 tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct
     1201 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     1261 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     1321 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     1381 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     1441 ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     1501 caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg
     1561 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     1621 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     1681 catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca
     1741 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     1801 tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     1861 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     1921 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     1981 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     2041 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     2101 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     2161 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     2221 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     2281 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     2341 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     2401 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     2461 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     2521 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     2581 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     2641 gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt
     2701 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     2761 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     2821 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     2881 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     2941 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     3001 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     3061 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     3121 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     3181 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     3241 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     3301 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3361 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3421 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3481 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3541 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3601 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3661 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3721 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3781 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3841 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3901 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3961 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     4021 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     4081 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4141 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4201 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4261 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4321 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4381 caattccgac g
//
