LOCUS       sequence-200522-        3944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mRuby3 Addgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mRuby3 Addgene
FEATURES             Location/Qualifiers
     source          1..3944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..69
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1002..1048
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1221..2171)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2219..2441
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2947..2981)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3022..3816)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3133..3152)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3743..3762
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggcc ctttcgtctt caccacagct aacaccacgt cgtccctatc tgctgcccta
      121 ggtctatgag tggttgctgg ataactttac gggcatgcat aaggctcgta tgatatattc
      181 agggagacca caacggtttc cctctacaaa taattttgtt taacttttct agatttaaga
      241 aggagatata catatgagta aaggagaaga actgatcaag gaaaatatgc gtatgaaggt
      301 ggtcatggaa ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag
      361 accgtacgag ggagtgcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt
      421 tgcctttgac attcttgcca cgtcgttcat gtatggcagc cgtaccttta tcaagtaccc
      481 ggccgacatc cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt
      541 tacgagatac gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg
      601 cgagctcgtc tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat
      661 gcagaagaag accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg
      721 tctgagagga tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa
      781 cttcgtgaca acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca
      841 tgccgttgat caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca
      901 aagagaagtg gcagttgcca aatacagcaa ccttggtggt ggcatggatg aactatacaa
      961 ataaatgtcc agacctgcag gcatgcaagc tctagaggca tcaaataaaa cgaaaggctc
     1021 agtcgaaaga ctgggccttt cgttttatct gttgaaatgc accaaaaact cgtaaaagct
     1081 ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg
     1141 atatctaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
     1201 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
     1261 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt actttgcatg
     1321 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
     1381 agtgtttttc ttagtccgtt acgtaggtag gaatctgatg taatggttgt tggtattttg
     1441 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
     1501 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
     1561 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
     1621 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
     1681 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
     1741 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
     1801 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
     1861 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
     1921 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
     1981 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
     2041 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
     2101 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
     2161 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
     2221 gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc
     2281 tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct
     2341 ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa
     2401 aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg
     2461 cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac
     2521 cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc
     2581 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     2641 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     2701 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     2761 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     2821 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     2881 aggcagcggt atcatcaaca ggcttacccg tcttactgtc aaccagaccc gccaggattc
     2941 tcatgcataa aaaacgcccg gcggcaaccg agcgttctga attaattaat catcgcgact
     3001 gcagagatcc cagagtcctt attagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3061 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3121 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3181 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3241 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     3301 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     3361 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     3421 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     3481 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     3541 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     3601 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     3661 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     3721 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     3781 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     3841 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     3901 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctg
//
