LOCUS       sequence-200523-        3929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mScarlet
FEATURES             Location/Qualifiers
     source          1..3929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..73
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      991..1037
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1210..2160)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2208..2430
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2936..2970)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3011..3805)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3122..3141)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3732..3751
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gctggcaatt ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
       61 cgtatcacga ggccctttcg tcttcaccac agctaacacc acgtcgtccc tatctgctgc
      121 cctaggtcta tgagtggttg ctggataact ttacgggcat gcataaggct cgtatgatat
      181 attcagggag accacaacgg tttccctcta caaataattt tgtttaactt ttctagattt
      241 aagaaggaga tatacatatg agtaaaggag aagctgtgat taaagagttc atgcgcttca
      301 aagttcacat ggagggttct atgaacggtc acgagttcga gatcgaaggc gaaggcgagg
      361 gccgtccgta tgaaggcacc cagaccgcca aactgaaagt gactaaaggc ggcccgctgc
      421 ctttttcctg ggacatcctg agcccgcaat ttatgtacgg ttctagggcg ttcaccaaac
      481 acccagcgga tatcccggac tattataagc agtcttttcc ggaaggtttc aagtgggaac
      541 gcgtaatgaa ttttgaagat ggtggtgccg tgaccgtcac tcaggacacc tccctggagg
      601 atggcaccct gatctataaa gttaaactgc gtggtactaa ttttccacct gatggcccgg
      661 tgatgcagaa aaagacgatg ggttgggagg cgtctaccga acgcttgtat ccggaagatg
      721 gtgtgctgaa aggcgacatt aaaatggccc tgcgcctgaa agatggcggc cgctatctgg
      781 ctgacttcaa aaccacgtac aaagccaaga aacctgtgca gatgcctggc gcgtacaatg
      841 tggaccgcaa actggacatc acctctcata atgaagatta tacggtggta gagcaatatg
      901 agcgctccga gggtcgtcat tctaccggtg gcatggatga actatacaaa taaatgtcca
      961 gacctgcagg catgcaagct ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     1021 tgggcctttc gttttatctg ttgaaatgca ccaaaaactc gtaaaagctc tgatgtatct
     1081 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg
     1141 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     1201 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     1261 ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa
     1321 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     1381 tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     1441 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     1501 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     1561 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     1621 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     1681 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     1741 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     1801 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     1861 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     1921 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     1981 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     2041 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     2101 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     2161 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     2221 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     2281 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     2341 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     2401 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     2461 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     2521 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     2581 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     2641 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     2701 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     2761 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     2821 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     2881 tcatcaacag gcttacccgt cttactgtca accagacccg ccaggattct catgcataaa
     2941 aaacgcccgg cggcaaccga gcgttctgaa ttaattaatc atcgcgactg cagagatccc
     3001 agagtcctta ttagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     3061 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     3121 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     3181 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     3241 catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     3301 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     3361 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     3421 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     3481 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     3541 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     3601 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     3661 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     3721 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     3781 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
     3841 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     3901 gcttcccaac cttaccagag ggcgcccca
//
